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Different features of Vδ2 T and NK cells in fatal and non-fatal human Ebola infections
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نویسنده
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cimini e. ,viola d. ,cabeza-cabrerizo m. ,romanelli a. ,tumino n. ,sacchi a. ,bordoni v. ,casetti r. ,turchi f. ,martini f. ,bore j.a. ,koundouno f.r. ,duraffour s. ,michel j. ,holm t. ,zekeng e.g. ,cowley l. ,garcia dorival i. ,doerrbecker j. ,hetzelt n. ,baum j.h.j. ,portmann j. ,wölfel r. ,gabriel m. ,miranda o. ,díaz g. ,díaz j.e. ,fleites y.a. ,piñeiro c.a. ,castro c.m. ,koivogui l. ,magassouba n. ,diallo b. ,ruibal p. ,oestereich l. ,wozniak d.m. ,lüdtke a. ,becker-ziaja b. ,capobianchi m.r. ,ippolito g. ,carroll m.w. ,günther s. ,di caro a. ,muñoz-fontela c. ,agrati c.
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منبع
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plos neglected tropical diseases - 2017 - دوره : 11 - شماره : 5
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چکیده
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Background: human ebola infection is characterized by a paralysis of the immune system. a signature of αβ t cells in fatal ebola infection has been recently proposed,while the involvement of innate immune cells in the protection/pathogenesis of ebola infection is unknown. aim of this study was to analyze γδ t and nk cells in patients from the ebola outbreak of 2014–2015 occurred in west africa,and to assess their association with the clinical outcome. methodology/principal findings: nineteen ebola-infected patients were enrolled at the time of admission to the ebola treatment centre in guinea. patients were divided in two groups on the basis of the clinical outcome. the analysis was performed by using multiparametric flow cytometry established by the european mobile laboratory in the field. a low frequency of vδ2 t-cells was observed during ebola infection,independently from the clinical outcome. moreover,vδ2 t-cells from ebola patients massively expressed cd95 apoptotic marker,suggesting the involvement of apoptotic mechanisms in vδ2 t-cell loss. interestingly,vδ2 t-cells from survivors expressed an effector phenotype and presented a lower expression of the ctla-4 exhaustion marker than fatalities,suggesting a role of effector vδ2 t-cells in the protection. furthermore,patients with fatal ebola infection were characterized by a lower nk cell frequency than patients with non fatal infection. in particular,both cd56brightand cd56dimnk frequency were very low both in fatal and non fatal infections,while a higher frequency of cd56negnk cells was associated to non-fatal infections. finally,nk activation and expression of nkp46 and cd158a were independent from clinical outcome. conclusions/significances: altogether,the data suggest that both effector vδ2 t-cells and nk cells may play a role in the complex network of protective response to ebov infection. further studies are required to characterize the protective effector functions of vδ2 and nk cells. © 2017 cimini et al.
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آدرس
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department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg, Germany, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, european mobile laboratory consortium,hamburg, Germany, european mobile laboratory consortium,hamburg, Germany, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg, Germany, european mobile laboratory consortium,hamburg,germany,robert koch institute,berlin, Germany, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg, Germany, european mobile laboratory consortium,hamburg,germany,institute of infection and global health,university of liverpool,liverpool, United Kingdom, european mobile laboratory consortium,hamburg,germany,national infection service,public health england,porton down and colindale, United Kingdom, european mobile laboratory consortium,hamburg,germany,institute of infection and global health,university of liverpool,liverpool, United Kingdom, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg,germany,centre for experimental and clinical infection research (twincore),institute for experimental virology,hannover, Germany, european mobile laboratory consortium,hamburg,germany,robert koch institute,berlin, Germany, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg, Germany, european mobile laboratory consortium,hamburg,germany,federal office for civil protection,spiez laboratory, Switzerland, european mobile laboratory consortium,hamburg,germany,bundeswehr institute of microbiology,munich,germany,german center for infection research (dzif),partner sites hamburg,munich, Germany, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg,germany,german center for infection research (dzif),partner sites hamburg,munich, Germany, hospital militar central dr. carlos j. finlay,havana, Cuba, hospital militar central dr. carlos j. finlay,havana, Cuba, hospital militar central dr. carlos j. finlay,havana, Cuba, hospital militar central dr. carlos j. finlay,havana, Cuba, hospital militar central dr. carlos j. finlay,havana, Cuba, hospital militar central dr. carlos j. finlay,havana, Cuba, institut national de santé publique,conakry, Guinea, laboratoire des fièvres hémorragiques en guinée,université gamal abdel nasser de conakry,conakry, Guinea, world health organization,geneva,switzerland,world health organization,conakry, Guinea, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg,germany,german center for infection research (dzif),partner sites hamburg,munich,germany,heinrich pette institute,leibniz institute for experimental virology,hamburg, Germany, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg,germany,german center for infection research (dzif),partner sites hamburg,munich, Germany, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg,germany,german center for infection research (dzif),partner sites hamburg,munich, Germany, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg,germany,german center for infection research (dzif),partner sites hamburg,munich,germany,heinrich pette institute,leibniz institute for experimental virology,hamburg, Germany, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg,germany,german center for infection research (dzif),partner sites hamburg,munich, Germany, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy, european mobile laboratory consortium,hamburg,germany,national infection service,public health england,porton down and colindale,united kingdom,university of southampton,south general hospital,southampton, United Kingdom, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg,germany,german center for infection research (dzif),partner sites hamburg,munich, Germany, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome,italy,european mobile laboratory consortium,hamburg, Germany, european mobile laboratory consortium,hamburg,germany,department of virology,bernhard nocht institute for tropical medicine,world health organization collaborating center for arbovirus and hemorrhagic fever reference and research,hamburg,germany,german center for infection research (dzif),partner sites hamburg,munich,germany,heinrich pette institute,leibniz institute for experimental virology,hamburg, Germany, department of epidemiology and pre-clinical research,national institute for infectious diseases lazzaro spallanzani,rome, Italy
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