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Point-of-care testing for Toxoplasma gondii IgG/IgM using Toxoplasma ICT IgG-IgM test with sera from the United States and implications for developing countries
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نویسنده
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begeman i.j. ,lykins j. ,zhou y. ,lai b.s. ,levigne p. ,el bissati k. ,boyer k. ,withers s. ,clouser f. ,noble a.g. ,rabiah p. ,swisher c.n. ,heydemann p.t. ,contopoulos-ioannidis d.g. ,montoya j.g. ,maldonado y. ,ramirez r. ,press c. ,stillwaggon e. ,peyron f. ,mcleod r.
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منبع
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plos neglected tropical diseases - 2017 - دوره : 11 - شماره : 6
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چکیده
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Background: congenital toxoplasmosis is a serious but preventable and treatable disease. gestational screening facilitates early detection and treatment of primary acquisition. thus,fetal infection can be promptly diagnosed and treated and outcomes can be improved. methods: we tested 180 sera with the toxoplasma ict igg-igm point-of-care (poc) test. sera were from 116 chronically infected persons (48 serotype ii; 14 serotype i-iii; 25 serotype i-iiia; 28 serotype atypical,haplogroup 12; 1 not typed). these represent strains of parasites infecting mothers of congenitally infected children in the u.s. 51 seronegative samples and 13 samples from recently infected persons known to be igg/igm positive within the prior 2.7 months also were tested. interpretation was confirmed by two blinded observers. a comparison of costs for poc vs. commercial laboratory testing methods was performed. results: we found that this new toxoplasma ict igg-igm poc test was highly sensitive (100%) and specific (100%) for distinguishing igg/igm-positive from negative sera. use of such reliable poc tests can be cost-saving and benefit patients. conclusions: our work demonstrates that the toxoplasma ict igg-igm test can function reliably as a point-of-care test to diagnose toxoplasma gondii infection in the u.s. this provides an opportunity to improve maternal-fetal care by using approaches,diagnostic tools,and medicines already available. this infection has serious,lifelong consequences for infected persons and their families. from the present study,it appears a simple,low-cost poc test is now available to help prevent morbidity/disability,decrease cost,and make gestational screening feasible. it also offers new options for improved prenatal care in low- and middle-income countries. © 2017 begeman et al.
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آدرس
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department of ophthalmology and visual science,the university of chicago,chicago,il, United States, pritzker school of medicine,the university of chicago,chicago,il, United States, department of ophthalmology and visual science,the university of chicago,chicago,il, United States, department of ophthalmology and visual science,the university of chicago,chicago,il, United States, institut de parasitologie et de mycologie médicale hôpital de la croix rousse,lyon, France, department of ophthalmology and visual science,the university of chicago,chicago,il, United States, department of ophthalmology and visual science,the university of chicago,chicago,il,united states,rush university and medical center,chicago,il, United States, department of ophthalmology and visual science,the university of chicago,chicago,il, United States, department of ophthalmology and visual science,the university of chicago,chicago,il, United States, department of ophthalmology and visual science,the university of chicago,chicago,il,united states,lurie children’s hospital and northwestern university,chicago,il, United States, department of ophthalmology and visual science,the university of chicago,chicago,il,united states,northshore hospital,evanston,il, United States, department of ophthalmology and visual science,the university of chicago,chicago,il,united states,lurie children’s hospital and northwestern university,chicago,il, United States, department of ophthalmology and visual science,the university of chicago,chicago,il,united states,rush university and medical center,chicago,il, United States, department of pediatrics,division of infectious diseases,stanford university school of medicine,stanford,ca, United States, palo alto medical foundation toxoplasma serology laboratory,palo alto,ca,united states,department of medicine,division of infectious diseases and geographic medicine,stanford university school of medicine,stanford,ca, United States, department of pediatrics,division of infectious diseases,stanford university school of medicine,stanford,ca,united states,department of health research and policy,stanford university school of medicine,stanford,ca, United States, palo alto medical foundation toxoplasma serology laboratory,palo alto,ca, United States, palo alto medical foundation toxoplasma serology laboratory,palo alto,ca, United States, gettysburg college,gettysburg,pa, United States, institut de parasitologie et de mycologie médicale hôpital de la croix rousse,lyon, France, department of ophthalmology and visual science,the university of chicago,chicago,il,united states,department of pediatrics (infectious diseases),institute of genomics,genetics and systems biology,global health center,toxoplasmosis center,the center for health and the social sciences,chess,the college,the university of chicago,chicago medicine,chicago,il, United States
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Authors
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