Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A

The helminth schistosoma mansoni modulates the infected host’s immune system to facilitate its own survival,by producing excretory/secretory molecules that interact with a variety of the host’s cell types including those of the immune system. herein,we characterise the s. mansoni adult male worm secretome and identify 111 proteins,including 7 vaccine candidates and several molecules with potential immunomodulatory activity. amongst the molecules present in the secretome,a 17-19kda protein analogous to human cyclophilin a was identified. given the ability of cyclophilin a to modulate the immune system by regulating antigen presenting cell activity,we sought to determine whether recombinant s. mansoni cyclophilin a (rsmcypa) is capable of modulating bone-marrow derived dendritic cell (bmdc) and t cell responses under in vitro conditions. rsmcypa was enzymatically active and able to alter the pro-inflammatory cytokine profile of lps-activated dendritic cells. rsmcypa also modulated dc function in the induction of cd4+t cell proliferation with a preferential expansion of treg cells. this work demonstrates the unique protein composition of the s. mansoni male worm secretome and immunomodulatory activity of s. mansoni cyclophilin a. © 2017 floudas et al.