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A High Resolution Case Study of a Patient with Recurrent Plasmodium vivax Infections Shows That Relapses Were Caused by Meiotic Siblings
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نویسنده
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bright a.t. ,manary m.j. ,tewhey r. ,arango e.m. ,wang t. ,schork n.j. ,yanow s.k. ,winzeler e.a.
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منبع
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plos neglected tropical diseases - 2014 - دوره : 8 - شماره : 6
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چکیده
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Plasmodium vivax infects a hundred million people annually and endangers 40% of the world's population. unlike plasmodium falciparum,p. vivax parasites can persist as a dormant stage in the liver,known as the hypnozoite,and these dormant forms can cause malaria relapses months or years after the initial mosquito bite. here we analyze whole genome sequencing data from parasites in the blood of a patient who experienced consecutive p. vivax relapses over 33 months in a non-endemic country. by analyzing patterns of identity,read coverage,and the presence or absence of minor alleles in the initial polyclonal and subsequent monoclonal infections,we show that the parasites in the three infections are likely meiotic siblings. we infer that these siblings are descended from a single tetrad-like form that developed in the infecting mosquito midgut shortly after fertilization. in this natural cross we find the recombination rate for p. vivax to be 10 kb per centimorgan and we further observe areas of disequilibrium surrounding major drug resistance genes. our data provide new strategies for studying multiclonal infections,which are common in all types of infectious diseases,and for distinguishing p. vivax relapses from reinfections in malaria endemic regions. this work provides a theoretical foundation for studies that aim to determine if new or existing drugs can provide a radical cure of p. vivax malaria. © 2014 bright et al.
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آدرس
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biomedical sciences program,school of medicine,university of california,san diego,la jolla,ca,united states,department of pediatrics,school of medicine,university of california,san diego,la jolla,ca, United States, biomedical sciences program,school of medicine,university of california,san diego,la jolla,ca,united states,department of pediatrics,school of medicine,university of california,san diego,la jolla,ca, United States, scripps genomic medicine,the scripps translational science institute,la jolla,ca,united states,department of molecular and experimental medicine,the scripps research institute,la jolla,ca, United States, grupo salud y comunidad,universidad de antioquia,medellín,antioquia, Colombia, biomedical sciences program,school of medicine,university of california,san diego,la jolla,ca, United States, scripps genomic medicine,the scripps translational science institute,la jolla,ca,united states,department of molecular and experimental medicine,the scripps research institute,la jolla,ca, United States, school of public health,university of alberta,edmonton,ab,canada,provincial laboratory for public health,edmonton,ab, Canada, department of pediatrics,school of medicine,university of california,san diego,la jolla,ca, United States
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Authors
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