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The Interface between Methyltransferase and Polymerase of NS5 Is Essential for Flavivirus Replication
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نویسنده
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li x.-d. ,shan c. ,deng c.-l. ,ye h.-q. ,shi p.-y. ,yuan z.-m. ,gong p. ,zhang b.
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منبع
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plos neglected tropical diseases - 2014 - دوره : 8 - شماره : 5
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چکیده
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The flavivirus ns5 harbors both a methyltransferase (mtase) and an rna-dependent rna polymerase (rdrp). both enzyme activities of ns5 are critical for viral replication. recently,the full-length ns5 crystal structure of japanese encephalitis virus reveals a conserved mtase-rdrp interface that features two conserved components: a six-residue hydrophobic network and a gtr sequence. here we showed for the first time that these key interface components are essential for flavivirus replication by various reverse genetics approaches. interestingly,some replication-impaired variants generated a common compensatory ns5 mutation outside the interface (l322f),providing novel routes to further explore the crosstalk between mtase and rdrp. © 2014 li et al.
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آدرس
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cas key laboratory of special pathogens and biosafety,center for emerging infectious diseases,wuhan institute of virology,chinese academy of sciences,wuhan,china,university of chinese academy of sciences,beijing, China, cas key laboratory of special pathogens and biosafety,center for emerging infectious diseases,wuhan institute of virology,chinese academy of sciences,wuhan,china,university of chinese academy of sciences,beijing, China, cas key laboratory of special pathogens and biosafety,center for emerging infectious diseases,wuhan institute of virology,chinese academy of sciences,wuhan,china,key laboratory of agricultural and environmental microbiology,wuhan institute of virology,chinese academy of sciences,wuhan, China, cas key laboratory of special pathogens and biosafety,center for emerging infectious diseases,wuhan institute of virology,chinese academy of sciences,wuhan,china,state key laboratory of virology,wuhan institute of virology,chinese academy of sciences,wuhan, China, wadsworth center,new york state department of health,albany,ny, United States, key laboratory of agricultural and environmental microbiology,wuhan institute of virology,chinese academy of sciences,wuhan, China, cas key laboratory of special pathogens and biosafety,center for emerging infectious diseases,wuhan institute of virology,chinese academy of sciences,wuhan,china,state key laboratory of virology,wuhan institute of virology,chinese academy of sciences,wuhan, China, cas key laboratory of special pathogens and biosafety,center for emerging infectious diseases,wuhan institute of virology,chinese academy of sciences,wuhan,china,key laboratory of agricultural and environmental microbiology,wuhan institute of virology,chinese academy of sciences,wuhan, China
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Authors
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