Dengue Virus Type 2 (DENV2)-Induced Oxidative Responses in Monocytes from Glucose-6-Phosphate Dehydrogenase (G6PD)-Deficient and G6PD Normal Subjects

Background:dengue virus is endemic in peninsular malaysia. the clinical manifestations vary depending on the incubation period of the virus as well as the immunity of the patients. glucose-6-phosphate dehydrogenase (g6pd) deficiency is prevalent in malaysia where the incidence is 3.2%. it has been noted that some g6pd-deficient individuals suffer from more severe clinical presentation of dengue infection. in this study,we aim to investigate the oxidative responses of denv2-infected monocytes from g6pd-deficient individuals.methodology:monocytes from g6pd-deficient individuals were infected with denv2 and infection rate,levels of oxidative species,nitric oxide (no),superoxide anions (o2 -),and oxidative stress were determined and compared with normal controls.principal findings:monocytes from g6pd-deficient individuals exhibited significantly higher infection rates compared to normal controls. in an effort to explain the reason for this enhanced susceptibility,we investigated the production of no and o2 - in the monocytes of individuals with g6pd deficiency compared with normal controls. we found that levels of no and o2 - were significantly lower in the denv-infected monocytes from g6pd-deficient individuals compared with normal controls. furthermore,the overall oxidative stress in denv-infected monocytes from g6pd-deficient individuals was significantly higher when compared to normal controls. correlation studies between denv-infected cells and oxidative state of monocytes further confirmed these findings.conclusions/significance:altered redox state of denv-infected monocytes from g6pd-deficient individuals appears to augment viral replication in these cells. denv-infected g6pd-deficient individuals may contain higher viral titers,which may be significant in enhanced virus transmission. furthermore,granulocyte dysfunction and higher viral loads in g6pd-deificient individuals may result in severe form of dengue infection. © 2014 al-alimi et al.