MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production

Micrornas have been shown to contribute to a repertoire of host-pathogen interactions during viral infection. our previous study demonstrated that microrna-30e* (mir-30e*) directly targeted the iκbα 3′-utr and disrupted the nf-κb/iκbα negative feedback loop,leading to hyperactivation of nf-κb. this current study investigated the possible role of mir-30e* in the regulation of innate immunity associated with dengue virus (denv) infection. we found that denv infection could induce mir-30e* expression in denv-permissive cells,and such an overexpression of mir-30e* upregulated ifn-β and the downstream ifn-stimulated genes (isgs) such as oas1,mxa and ifitm1,and suppressed denv replication. furthermore,suppression of iκbα mediates the enhancing effect of mir-30e* on ifn-β-induced antiviral response. collectively,our findings suggest a modulatory role of mir-30e* in denv induced ifn-β signaling via the nf-κb-dependent pathway. further investigation is needed to evaluate whether mir-30e* has an anti-denv effect in vivo. © 2014 zhu et al.