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Biodistribution and Toxicity Studies of PRINT Hydrogel Nanoparticles in Mosquito Larvae and Cells
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نویسنده
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phanse y. ,dunphy b.m. ,perry j.l. ,airs p.m. ,paquette c.c.h. ,carlson j.o. ,xu j. ,luft j.c. ,desimone j.m. ,beaty b.j. ,bartholomay l.c.
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منبع
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plos neglected tropical diseases - 2015 - دوره : 9 - شماره : 5
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چکیده
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Mosquito-borne diseases continue to remain major threats to human and animal health and impediments to socioeconomic development. increasing mosquito resistance to chemical insecticides is a great public health concern,and new strategies/technologies are necessary to develop the next-generation of vector control tools. we propose to develop a novel method for mosquito control that employs nanoparticles (nps) as a platform for delivery of mosquitocidal dsrna molecules to silence mosquito genes and cause vector lethality. identifying optimal np chemistry and morphology is imperative for efficient mosquitocide delivery. toward this end,fluorescently labeled polyethylene glycol nps of specific sizes,shapes (80 nm x 320 nm,80 nm x 5000 nm,200 nm x 200 nm,and 1000 nm x 1000 nm) and charges (negative and positive) were fabricated by particle replication in non-wetting templates (print) technology. biodistribution,persistence,and toxicity of print nps were evaluated in vitro in mosquito cell culture and in vivo in anopheles gambiae larvae following parenteral and oral challenge. following parenteral challenge,the biodistribution of the positively and negatively charged nps of each size and shape was similar; intense fluorescence was observed in thoracic and abdominal regions of the larval body. positively charged nps were more associated with the gastric caeca in the gastrointestinal tract. negatively charged nps persisted through metamorphosis and were observed in head,body and ovaries of adults. following oral challenge,nps were detected in the larval mid- and hindgut. positively charged nps were more efficiently internalized in vitro than negatively charged nps. positively charged nps trafficked to the cytosol,but negatively charged nps co-localized with lysosomes. following in vitro and in vivo challenge,none of the nps tested induced any cytotoxic effects. © 2015 phanse et al.
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آدرس
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department of entomology,iowa state university,ames,ia, United States, department of entomology,iowa state university,ames,ia, United States, lineberger comprehensive cancer center,university of north carolina at chapel hillnc, United States, department of entomology,iowa state university,ames,ia, United States, department of microbiology,immunology,and pathology,colorado state university,fort collins,co, United States, department of microbiology,immunology,and pathology,colorado state university,fort collins,co, United States, lineberger comprehensive cancer center,university of north carolina at chapel hillnc, United States, lineberger comprehensive cancer center,university of north carolina at chapel hillnc,united states,eshelman school of pharmacy,university of north carolina at chapel hill,chapel hill,nc, United States, lineberger comprehensive cancer center,university of north carolina at chapel hillnc,united states,eshelman school of pharmacy,university of north carolina at chapel hill,chapel hill,nc,united states,department of chemistry,university of north carolina at chapel hill,chapel hill,nc,united states,institute for nanomedicine and institute for advanced materials,university of north carolina at chapel hill,chapel hill,nc,united states,department of chemical and biomolecular engineering,north carolina state university,raleigh,nc,united states,sloan-kettering institute for cancer research,memorial sloan-kettering cancer center,new york,ny, United States, department of microbiology,immunology,and pathology,colorado state university,fort collins,co, United States, department of entomology,iowa state university,ames,ia, United States
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Authors
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