Proteomics-based characterization of the humoral immune response in sporotrichosis: Toward discovery of potential diagnostic and vaccine antigens

Background sporothrix schenckii and associated species are agents of human and animal sporotrichosis that cause large sapronoses and zoonoses worldwide. epidemiological surveillance has highlighted an overwhelming occurrence of the highly pathogenic fungus sporothrix brasiliensis during feline outbreaks,leading to massive transmissions to humans. early diagnosis of feline sporotrichosis by demonstrating the presence of a surrogate marker of infection can have a key role for selecting appropriate disease control measures and minimizing zoonotic transmission to humans. methodology we explored the presence and diversity of serum antibodies (igg) specific against sporothrix antigens in cats with sporotrichosis and evaluated the utility of these antibodies for serodiagnosis. antigen profiling included protein extracts from the closest known relatives s. brasiliensis and s. schenckii. enzyme-linked immunosorbent assays and immunoblotting enabled us to characterize the major antigens of feline sporotrichosis from sera from cats with sporotrichosis (n = 49),healthy cats (n = 19),and cats with other diseases (n = 20). principal findings enzyme-linked immunosorbent assay-based quantitation of anti-sporothrix igg exhibited high sensitivity and specificity in cats with sporotrichosis (area under the curve,1.0; 95% confidence interval,0.94–1; p<0.0001) versus controls. the two sets of sporothrix antigens were remarkably cross-reactive,supporting the hypothesis that antigenic epitopes may be conserved among closely related agents. one-dimensional immunoblotting indicated that 3-carboxymuconate cyclase (a 60-kda protein in s. brasiliensis and a 70-kda protein in s. schenckii) is the immunodominant antigen in feline sporotrichosis. two-dimensional immunoblotting revealed six igg-reactive isoforms of gp60 in the s. brasiliensis proteome,similar to the humoral response found in human sporotrichosis. © 2015 rodrigues et al.