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Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection
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نویسنده
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erasmus j.h. ,needham j. ,raychaudhuri s. ,diamond m.s. ,beasley d.w.c. ,morkowski s. ,salje h. ,fernandez salas i. ,kim d.y. ,frolov i. ,nasar f. ,weaver s.c.
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منبع
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plos neglected tropical diseases - 2015 - دوره : 9 - شماره : 10
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چکیده
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In december of 2013,chikungunya virus (chikv),an alphavirus in the family togaviridae,was introduced to the island of saint martin in the caribbean,resulting in the first autochthonous cases reported in the americas. as of january 2015,local and imported chikv has been reported in 50 american countries with over 1.1 million suspected cases. chikv causes a severe arthralgic disease for which there are no approved vaccines or therapeutics. furthermore,the lack of a commercially available,sensitive,and affordable diagnostic assay limits surveillance and control efforts. to address this issue,we utilized an insect-specific alphavirus,eilat virus (eilv),to develop a diagnostic antigen that does not require biosafety containment facilities to produce. we demonstrated that eilv/chikv replicates to high titers in insect cells and can be applied directly in enzyme-linked immunosorbent assays without inactivation,resulting in highly sensitive detection of recent and past chikv infection,and outperforming traditional antigen preparations. © 2015,public library of science. all rights reserved.
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آدرس
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institute for translational sciences,university of texas medical branch,galveston,tx,united states,center for tropical diseases,university of texas medical branch,galveston,tx, United States, inbios international,inc.,seattle,wa, United States, inbios international,inc.,seattle,wa, United States, departments of medicine,molecular microbiology,pathology & immunology,washington university school of medicine,st. louis,mo, United States, institute for human infections and immunity,department of microbiology and immunology,university of texas medical branch,galveston,tx,united states,center for biodefense and emerging infectious diseases,and sealy center for vaccine development,university of texas medical branch,galveston,tx, United States, inbios international,inc.,seattle,wa, United States, johns hopkins bloomberg school of public health,baltimore,md,united states,pasteur institute,paris, France, centro regional de investigación en salud publica insp.,tapachula,chiapas, Mexico, department of microbiology,university of alabama at birmingham,birmingham,al, United States, department of microbiology,university of alabama at birmingham,birmingham,al, United States, institute for translational sciences,university of texas medical branch,galveston,tx,united states,center for tropical diseases,university of texas medical branch,galveston,tx,united states,virology division,united states army medical research institute of infectious diseases,frederick,md, United States, institute for translational sciences,university of texas medical branch,galveston,tx,united states,center for tropical diseases,university of texas medical branch,galveston,tx,united states,institute for human infections and immunity,department of microbiology and immunology,university of texas medical branch,galveston,tx, United States
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Authors
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