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Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
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نویسنده
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housman g. ,malukiewicz j. ,boere v. ,grativol a.d. ,pereira l.c.m. ,silva i.d.o.e. ,ruiz-miranda c.r. ,truman r. ,stone a.c.
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منبع
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plos neglected tropical diseases - 2015 - دوره : 9 - شماره : 11
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چکیده
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Zoonotic pathogens that cause leprosy (mycobacterium leprae) and tuberculosis (mycobacterium tuberculosis complex,mtbc) continue to impact modern human populations. therefore,methods able to survey mycobacterial infection in potential animal hosts are necessary for proper evaluation of human exposure threats. here we tested for mycobacterial-specific single- and multi-copy loci using qpcr. in a trial study in which armadillos were artificially infected with m. leprae,these techniques were specific and sensitive to pathogen detection,while more traditional elisas were only specific. these assays were then employed in a case study to detect m. leprae as well as mtbc in wild marmosets. all marmosets were negative for m. leprae dna,but 14 were positive for the mycobacterial rpob gene assay. targeted capture and sequencing of rpob and other mtbc genes validated the presence of mycobacterial dna in these samples and revealed that qpcr is useful for identifying mycobacterial-infected animal hosts. © 2015,public library of science. all rights reserved.
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آدرس
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school of human evolution and social change,arizona state university,tempe,az, United States, school of human evolution and social change,arizona state university,tempe,az,united states,departamento de bioquímica e biologia molecular,universidade federal de viçosa,viçosa,minas gerais, Brazil, departamento de bioquímica e biologia molecular,universidade federal de viçosa,viçosa,minas gerais, Brazil, laboratório de ciências ambientias,centro de biociências e biotecnologia,universidade estadual do norte fluminense,campos dos goytacazes,rio de janeiro, Brazil, centro de conservação e manejo de fauna da caatinga,universidade federal do vale do são francisco,petrolina,pernambuco, Brazil, departamento de biologia animal,universidade federal de viçosa,viçosa,minas gerais, Brazil, laboratório de ciências ambientias,centro de biociências e biotecnologia,universidade estadual do norte fluminense,campos dos goytacazes,rio de janeiro, Brazil, hhshrsahsb national hansen’s disease program-niaid,baton rouge,la iaa-2646, United States, school of human evolution and social change,arizona state university,tempe,az, United States
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Authors
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