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The use of a mobile laboratory unit in support of patient management and epidemiological surveillance during the 2005 Marburg outbreak in Angola
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نویسنده
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grolla a. ,jones s.m. ,fernando l. ,strong j.e. ,ströher u. ,möller p. ,paweska j.t. ,burt f. ,palma p. ,sprecher a. ,formenty p. ,roth c. ,feldmann h.
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منبع
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plos neglected tropical diseases - 2011 - دوره : 5 - شماره : 5
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چکیده
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Background: marburg virus (marv),a zoonotic pathogen causing severe hemorrhagic fever in man,has emerged in angola resulting in the largest outbreak of marburg hemorrhagic fever (mhf) with the highest case fatality rate to date. methodology/principal findings: a mobile laboratory unit (mlu) was deployed as part of the world health organization outbreak response. utilizing quantitative real-time pcr assays,this laboratory provided specific marv diagnostics in uige,the epicentre of the outbreak. the mlu operated over a period of 88 days and tested 620 specimens from 388 individuals. specimens included mainly oral swabs and edta blood. following establishing on site,the mlu operation allowed a diagnostic response in <4 hours from sample receiving. most cases were found among females in the child-bearing age and in children less than five years of age. the outbreak had a high number of paediatric cases and breastfeeding may have been a factor in marv transmission as indicated by the epidemiology and marv positive breast milk specimens. oral swabs were a useful alternative specimen source to whole blood/serum allowing testing of patients in circumstances of resistance to invasive procedures but limited diagnostic testing to molecular approaches. there was a high concordance in test results between the mlu and the reference laboratory in luanda operated by the us centers for disease control and prevention. conclusions/significance: the mlu was an important outbreak response asset providing support in patient management and epidemiological surveillance. field laboratory capacity should be expanded and made an essential part of any future outbreak investigation.
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آدرس
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special pathogens program,national microbiology laboratory,public health agency of canada,winnipeg,mb, Canada, special pathogens program,national microbiology laboratory,public health agency of canada,winnipeg,mb,canada,department of medical microbiology,university of manitoba,winnipeg,mb,canada,department of immunology,university of manitoba,winnipeg,mb, Canada, special pathogens program,national microbiology laboratory,public health agency of canada,winnipeg,mb, Canada, special pathogens program,national microbiology laboratory,public health agency of canada,winnipeg,mb,canada,department of medical microbiology,university of manitoba,winnipeg,mb,canada,department of pediatrics and child health,university of manitoba,winnipeg,mb, Canada, special pathogens program,national microbiology laboratory,public health agency of canada,winnipeg,mb,canada,department of medical microbiology,university of manitoba,winnipeg,mb, Canada, institut für virologie,philipps-universität,marburg,hessen,germany,novartis vaccines and diagnostics,amsterdam, Netherlands, special pathogens unit,national institute for communicable diseases,national health laboratory service,sandringham, South Africa, special pathogens unit,national institute for communicable diseases,national health laboratory service,sandringham,south africa,department of medical virology,national health laboratory services universitas,university of the free state,bloemfontein, South Africa, médecins sans frontières,barcelona, Spain, médecins sans frontières,brussels, Belgium, world health organization,geneva, Switzerland, world health organization,geneva, Switzerland, special pathogens program,national microbiology laboratory,public health agency of canada,winnipeg,mb,canada,department of medical microbiology,university of manitoba,winnipeg,mb,canada,laboratory of virology,division of intramural research,national institute of allergy and infectious diseases,national institutes of health,hamilton,mt, United States
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Authors
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