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Developmental transcriptomic features of the Carcinogenic Liver Fluke,Clonorchis sinensis
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نویسنده
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yoo w.g. ,kim d.-w. ,ju j.-w. ,cho p.y. ,kim t.i. ,cho s.-h. ,choi s.-h. ,park h.-s. ,kim t.-s. ,hong s.-j.
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منبع
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plos neglected tropical diseases - 2011 - دوره : 5 - شماره : 6
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چکیده
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Clonorchis sinensis is the causative agent of the life-threatening disease endemic to china,korea,and vietnam. it is estimated that about 15 million people are infected with this fluke. c. sinensis provokes inflammation,epithelial hyperplasia,and periductal fibrosis in bile ducts,and may cause cholangiocarcinoma in chronically infected individuals. accumulation of a large amount of biological information about the adult stage of this liver fluke in recent years has advanced our understanding of the pathological interplay between this parasite and its hosts. however,no developmental gene expression profiles of c. sinensis have been published. in this study,we generated gene expression profiles of three developmental stages of c. sinensis by analyzing expressed sequence tags (ests). complementary dna libraries were constructed from the adult,metacercaria,and egg developmental stages of c. sinensis. a total of 52,745 ests were generated and assembled into 12,830 c. sinensis assembled est sequences,and then these assemblies were further categorized into groups according to biological functions and developmental stages. most of the genes that were differentially expressed in the different stages were consistent with the biological and physical features of the particular developmental stage; high energy metabolism,motility and reproduction genes were differentially expressed in adults,minimal metabolism and final host adaptation genes were differentially expressed in metacercariae,and embryonic genes were differentially expressed in eggs. the higher expression of glucose transporters,proteases,and antioxidant enzymes in the adults accounts for active uptake of nutrients and defense against host immune attacks. the types of ion channels present in c. sinensis are consistent with its parasitic nature and phylogenetic placement in the tree of life. we anticipate that the transcriptomic information on essential regulators of development,bile chemotaxis,and physico-metabolic pathways in c. sinensis that presented in this study will guide further studies to identify novel drug targets and diagnostic antigens. © 2011 yoo et al.
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آدرس
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department of medical environmental biology,research center for biomolecules and biosystems,chung-ang university college of medicine,seoul,south korea,division of malaria and parasitic diseases,national institute of health,korea centers for disease control and prevention,osong,chungbuk, South Korea, division of malaria and parasitic diseases,national institute of health,korea centers for disease control and prevention,osong,chungbuk,south korea,genome research center,korea research institute of bioscience and biotechnology,university of science and technology,daejeon, South Korea, division of malaria and parasitic diseases,national institute of health,korea centers for disease control and prevention,osong,chungbuk, South Korea, department of infection biology,zoonosis research center,wonkwang university school of medicine,chonbuk, South Korea, department of medical environmental biology,research center for biomolecules and biosystems,chung-ang university college of medicine,seoul, South Korea, division of malaria and parasitic diseases,national institute of health,korea centers for disease control and prevention,osong,chungbuk, South Korea, genome research center,korea research institute of bioscience and biotechnology,university of science and technology,daejeon, South Korea, genome research center,korea research institute of bioscience and biotechnology,university of science and technology,daejeon, South Korea, department of parasitology,inha university school of medicine,incheon, South Korea, department of medical environmental biology,research center for biomolecules and biosystems,chung-ang university college of medicine,seoul, South Korea
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Authors
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