|
|
|
|
Prevalence and factors associated with Leishmania infantum infection of dogs from an urban area of Brazil as identified by molecular methods
|
|
|
|
|
|
|
|
نویسنده
|
coura-vital w. ,marques m.j. ,veloso v.m. ,roatt b.m. ,de oliveira aguiar-soares r.d. ,reis l.e.s. ,braga s.l. ,morais m.h.f. ,reis a.b. ,carneiro m.
|
|
منبع
|
plos neglected tropical diseases - 2011 - دوره : 5 - شماره : 8
|
|
چکیده
|
Background: various factors contribute to the urbanization of the visceral leishmaniasis (vl),including the difficulties of implementing control measures relating to the domestic reservoir. the aim of this study was to determine the prevalence of canine visceral leishmaniasis in an urban endemic area in brazil and the factors associated with leishmania infantum infection among seronegative and pcr-positive dogs. methodology: a cross-sectional study was conducted in belo horizonte,minas gerais,brazil. blood samples were collected from 1,443 dogs. serology was carried out by using two enzyme-linked immunosorbent assays (biomanguinhos/fiocruz/rj and in house),and molecular methods were developed,including pcr-rflp. to identify the factors associated with early stages of infection,only seronegative (n = 1,213) animals were evaluated. these animals were divided into two groups: pcr-positive (n = 296) and pcr-negative (n = 917) for l. infantum dna. a comparison of these two groups of dogs taking into consideration the characteristics of the animals and their owners was performed. a mixed logistic regression model was used to identify factors associated with l. infantum infection. principal findings: of the 1,443 dogs examined,230 (15.9%) were seropositive in at least one elisa,whereas pcr-rflp revealed that 356 animals (24.7%) were positive for l. infantum dna. results indicated that the associated factors with infection were family income
|
|
|
|
|
آدرس
|
departamento de parasitologia,instituto de ciências biológicas,universidade federal de minas gerais,belo horizonte,minas gerais,brazil,núcleo de pesquisas em ciências biológicas,instituto de ciências exatas e biológicas,universidade federal de ouro preto,ouro preto,minas gerais, Brazil, departamento de ciências biológicas,universidade federal de alfenas,alfenas,minas gerais, Brazil, departamento de farmácia,escola de farmácia,universidade federal de ouro preto,ouro preto,minas gerais, Brazil, núcleo de pesquisas em ciências biológicas,instituto de ciências exatas e biológicas,universidade federal de ouro preto,ouro preto,minas gerais, Brazil, núcleo de pesquisas em ciências biológicas,instituto de ciências exatas e biológicas,universidade federal de ouro preto,ouro preto,minas gerais, Brazil, núcleo de pesquisas em ciências biológicas,instituto de ciências exatas e biológicas,universidade federal de ouro preto,ouro preto,minas gerais, Brazil, núcleo de pesquisas em ciências biológicas,instituto de ciências exatas e biológicas,universidade federal de ouro preto,ouro preto,minas gerais, Brazil, departamento de parasitologia,instituto de ciências biológicas,universidade federal de minas gerais,belo horizonte,minas gerais,brazil,gerência regional de controle de zoonoses,secretaria municipal de saúde,prefeitura de belo horizonte,belo horizonte,minas gerais, Brazil, núcleo de pesquisas em ciências biológicas,instituto de ciências exatas e biológicas,universidade federal de ouro preto,ouro preto,minas gerais, Brazil, departamento de parasitologia,instituto de ciências biológicas,universidade federal de minas gerais,belo horizonte,minas gerais,brazil,pós-graduação em infectologia e medicina tropical,faculdade de medicina,universidade federal de minas gerais,belo horizonte,minas gerais, Brazil
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|