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A ligA three-domain region protects hamsters from lethal infection by leptospira interrogans
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نویسنده
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coutinho m.l. ,choy h.a. ,kelley m.m. ,matsunaga j. ,babbitt j.t. ,lewis m.s. ,aleixo j.a.g. ,haake d.a.
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منبع
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plos neglected tropical diseases - 2011 - دوره : 5 - شماره : 12
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چکیده
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The leptospiral liga protein consists of 13 bacterial immunoglobulin-like (big) domains and is the only purified recombinant subunit vaccine that has been demonstrated to protect against lethal challenge by a clinical isolate of leptospira interrogans in the hamster model of leptospirosis. we determined the minimum number and location of liga domains required for immunoprotection. immunization with domains 11 and 12 was found to be required but insufficient for protection. inclusion of a third domain,either 10 or 13,was required for 100% survival after intraperitoneal challenge with leptospira interrogans serovar copenhageni strain fiocruz l1-130. as in previous studies,survivors had renal colonization; here,we quantitated the leptospiral burden by qpcr to be 1.2×10 3 to 8×10 5 copies of leptospiral dna per microgram of kidney dna. although renal histopathology in survivors revealed tubulointerstitial changes indicating an inflammatory response to the infection,blood chemistry analysis indicated that renal function was normal. these studies define the big domains of liga that account for its vaccine efficacy and highlight the need for additional strategies to achieve sterilizing immunity to protect the mammalian host from leptospiral infection and its consequences.
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آدرس
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veterans affairs greater los angeles healthcare system,los angeles,ca,united states,centro de desenvolvimento tecnologico,universidade federal de pelotas,pelotas, Brazil, veterans affairs greater los angeles healthcare system,los angeles,ca,united states,department of medicine,david geffen school of medicine,university of california los angeles (ucla),los angeles,ca, United States, veterans affairs greater los angeles healthcare system,los angeles,ca, United States, veterans affairs greater los angeles healthcare system,los angeles,ca,united states,department of medicine,david geffen school of medicine,university of california los angeles (ucla),los angeles,ca, United States, veterans affairs greater los angeles healthcare system,los angeles,ca,united states,department of medicine,david geffen school of medicine,university of california los angeles (ucla),los angeles,ca, United States, veterans affairs greater los angeles healthcare system,los angeles,ca, United States, centro de desenvolvimento tecnologico,universidade federal de pelotas,pelotas, Brazil, veterans affairs greater los angeles healthcare system,los angeles,ca,united states,department of medicine,david geffen school of medicine,university of california los angeles (ucla),los angeles,ca,united states,department of urology,david geffen school of medicine at ucla,los angeles,ca,united states,department of microbiology,immunology and molecular genetics,ucla,los angeles,ca, United States
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Authors
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