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A Hamster-Derived West Nile Virus Isolate Induces Persistent Renal Infection in Mice
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نویسنده
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saxena v. ,xie g. ,li b. ,farris t. ,welte t. ,gong b. ,boor p. ,wu p. ,tang s.-j. ,tesh r. ,wang t.
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منبع
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plos neglected tropical diseases - 2013 - دوره : 7 - شماره : 6
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چکیده
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Background:west nile virus (wnv) can persist long term in the brain and kidney tissues of humans,non-human primates,and hamsters. in this study,mice were infected with wnv strain h8912,previously cultured from the urine of a persistently infected hamster,to determine its pathogenesis in a murine host.methodology/principal findings:we found that wnv h8912 was highly attenuated for neuroinvasiveness in mice. following a systemic infection,viral rna could be detected quickly in blood and spleen and much later in kidneys. wnv h8912 induced constitutive il-10 production,upregulation of ifn-β and il-1β expression,and a specific igm response on day 10 post-infection. wnv h8912 persisted preferentially in kidneys with mild renal inflammation,and less frequently in spleen for up to 2.5 months post infection. this was concurrent with detectable serum wnv-specific igm and igg production. there were also significantly fewer wnv- specific t cells and lower inflammatory responses in kidneys than in spleen. previous studies have shown that systemic wild-type wnv ny99 infection induced virus persistence preferentially in spleen than in mouse kidneys. here,we noted that splenocytes of wnv h8912-infected mice produced significantly less il-10 than those of wnv ny99-infected mice. finally,wnv h8912 was also attenuated in neurovirulence. following intracranial inoculation,wnv persisted in the brain at a low frequency,concurrent with neither inflammatory responses nor neuronal damage in the brain.conclusions:wnv h8912 is highly attenuated in both neuroinvasiveness and neurovirulence in mice. it induces a low and delayed anti-viral response in mice and preferentially persists in the kidneys. © 2013 saxena et al.
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آدرس
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department of microbiology and immunology,the university of texas medical branch,galveston,tx, United States, department of microbiology and immunology,the university of texas medical branch,galveston,tx, United States, department of neuroscience and cell biology,the university of texas medical branch,galveston,tx, United States, department of microbiology and immunology,the university of texas medical branch,galveston,tx, United States, department of microbiology and immunology,the university of texas medical branch,galveston,tx, United States, department of pathology,the university of texas medical branch,galveston,tx, United States, department of pathology,the university of texas medical branch,galveston,tx, United States, department of neuroscience and cell biology,the university of texas medical branch,galveston,tx, United States, department of neuroscience and cell biology,the university of texas medical branch,galveston,tx, United States, department of pathology,the university of texas medical branch,galveston,tx,united states,center for biodefense and emerging infectious diseases,the university of texas medical branch,galveston,tx,united states,sealy center for vaccine development,the university of texas medical branch,galveston,tx, United States, department of microbiology and immunology,the university of texas medical branch,galveston,tx,united states,department of pathology,the university of texas medical branch,galveston,tx,united states,center for biodefense and emerging infectious diseases,the university of texas medical branch,galveston,tx,united states,sealy center for vaccine development,the university of texas medical branch,galveston,tx, United States
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Authors
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