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A Single Multilocus Sequence Typing (MLST) Scheme for Seven Pathogenic Leptospira Species
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نویسنده
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boonsilp s. ,thaipadungpanit j. ,amornchai p. ,wuthiekanun v. ,bailey m.s. ,holden m.t.g. ,zhang c. ,jiang x. ,koizumi n. ,taylor k. ,galloway r. ,hoffmaster a.r. ,craig s. ,smythe l.d. ,hartskeerl r.a. ,day n.p. ,chantratita n. ,feil e.j. ,aanensen d.m. ,spratt b.g. ,peacock s.j.
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منبع
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plos neglected tropical diseases - 2013 - دوره : 7 - شماره : 1
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چکیده
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Background: the available leptospira multilocus sequence typing (mlst) scheme supported by a mlst website is limited to l. interrogans and l. kirschneri. our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenic species. methodology and findings: we modified the existing scheme by replacing one of the seven mlst loci (fadd was changed to caib),as the former gene did not appear to be present in some pathogenic species. comparison of the original and modified schemes using data for l. interrogans and l. kirschneri demonstrated that the discriminatory power of the two schemes was not significantly different. the modified scheme was used to further characterize 325 isolates (l. alexanderi [n = 5],l. borgpetersenii [n = 34],l. interrogans [n = 222],l. kirschneri [n = 29],l. noguchii [n = 9],l. santarosai [n = 10],and l. weilii [n = 16]). phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each species corresponded to a discrete clade,and that no strains were misclassified at the species level. comparison between genotype and serovar was possible for 254 isolates. of the 31 sequence types (sts) represented by at least two isolates,18 sts included isolates assigned to two or three different serovars. conversely,14 serovars were identified that contained between 2 to 10 different sts. new observations were made on the global phylogeography of leptospira spp.,and the utility of mlst in making associations between human disease and specific maintenance hosts was demonstrated. conclusion: the new mlst scheme,supported by an updated mlst website,allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.
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آدرس
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mahidol-oxford tropical medicine research unit,faculty of tropical medicine,mahidol university,bangkok,thailand,department of microbiology and immunology,faculty of tropical medicine,mahidol university,bangkok, Thailand, mahidol-oxford tropical medicine research unit,faculty of tropical medicine,mahidol university,bangkok, Thailand, mahidol-oxford tropical medicine research unit,faculty of tropical medicine,mahidol university,bangkok, Thailand, mahidol-oxford tropical medicine research unit,faculty of tropical medicine,mahidol university,bangkok, Thailand, department of infection and tropical medicine,birmingham heartlands hospital,west midlands, United Kingdom, pathogen genomics,wellcome trust sanger institute,cambridge, United Kingdom, department of leptospirosis,national institute for communicable disease control and prevention,chinese center for disease control and prevention,beijing, China, department of leptospirosis,national institute for communicable disease control and prevention,chinese center for disease control and prevention,beijing, China, department of bacteriology,national institute of infectious diseases,tokyo, Japan, laboratory of wildlife biology and medicine,graduate school of veterinary medicine,hokkaido university,sapporo, Japan, national center for emerging and zoonotic infectious diseases,centers for diseases control and prevention,atlanta,ga, United States, national center for emerging and zoonotic infectious diseases,centers for diseases control and prevention,atlanta,ga, United States, who/fao/oie collaborating center for reference and research on leptospirosis,queensland health forensic and scientific services,health services support agency,queensland health,brisbane,qld, Australia, who/fao/oie collaborating center for reference and research on leptospirosis,queensland health forensic and scientific services,health services support agency,queensland health,brisbane,qld, Australia, who/fao/oie and national leptospirosis reference centre,kit biomedical research,royal tropical institute,amsterdam, Netherlands, mahidol-oxford tropical medicine research unit,faculty of tropical medicine,mahidol university,bangkok,thailand,center for tropical medicine,nuffield department of clinical medicine,university of oxford,churchill hospital,oxford, United Kingdom, mahidol-oxford tropical medicine research unit,faculty of tropical medicine,mahidol university,bangkok,thailand,department of microbiology and immunology,faculty of tropical medicine,mahidol university,bangkok, Thailand, department of biology and biochemistry,university of bath,bath, United Kingdom, department of infectious disease epidemiology,imperial college london,london, United Kingdom, department of infectious disease epidemiology,imperial college london,london, United Kingdom, mahidol-oxford tropical medicine research unit,faculty of tropical medicine,mahidol university,bangkok,thailand,department of microbiology and immunology,faculty of tropical medicine,mahidol university,bangkok,thailand,department of medicine,university of cambridge,addenbrooke's hospital,cambridge, United Kingdom
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Authors
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