|
|
|
|
Combined TLR7/8 and TLR9 Ligands Potentiate the Activity of a Schistosoma japonicum DNA Vaccine
|
|
|
|
|
|
|
|
نویسنده
|
wang x. ,dong l. ,ni h. ,zhou s. ,xu z. ,hoellwarth j.s. ,chen x. ,zhang r. ,chen q. ,liu f. ,wang j. ,su c.
|
|
منبع
|
plos neglected tropical diseases - 2013 - دوره : 7 - شماره : 4
|
|
چکیده
|
Background: toll-like receptor (tlr) ligands have been explored as vaccine adjuvants for tumor and virus immunotherapy,but few tlr ligands affecting schistosoma vaccines have been characterized. previously,we developed a partially protective dna vaccine encoding the 26-kda glutathione s-transferase of schistosoma japonicum (pvax1-sj26gst). methodology/principal findings: in this study,we evaluated a tlr7/8 ligand (r848) and a tlr9 ligand (cpg oligodeoxynucleotides,or cpg) as adjuvants for pvax1-sj26gst and assessed their effects on the immune system and protection against s. japonicum. we show that combining cpg and r848 with pvax1-sj26gst immunization significantly increases splenocyte proliferation and igg and igg2a levels,decreases cd4+cd25+foxp3+ regulatory t cells (treg) frequency in vivo,and enhances protection against s. japonicum. cpg and r848 inhibited treg-mediated immunosuppression,upregulated the production of interferon (ifn)-γ,tumor necrosis factor (tnf)-α,interleukin (il)-4,il-10,il-2,and il-6,and decreased foxp3 expression in vitro,which may contribute to prevent treg suppression and conversion during vaccination and allow expansion of antigen-specific t cells against pathogens. conclusions: our data shows that selective tlr ligands can increase the protective efficacy of dna vaccines against schistosomiasis,potentially through combined antagonism of treg-mediated immunosuppression and conversion. © 2013 wang et al.
|
|
|
|
|
آدرس
|
department of central laboratory,the affiliated people's hospital,jiangsu university,zhenjiang,jiangsu,china,department of pathogen biology and immunology,school of medicine,anhui university of science and technology,huainan,anhui, China, department of central laboratory,the affiliated people's hospital,jiangsu university,zhenjiang,jiangsu, China, department of central laboratory,the affiliated people's hospital,jiangsu university,zhenjiang,jiangsu, China, department of pathogen biology and immunology,jiangsu key laboratory of pathogen biology,nanjing medical university,nanjing,jiangsu, China, department of pathogen biology and immunology,jiangsu key laboratory of pathogen biology,nanjing medical university,nanjing,jiangsu, China, department of general surgery,keck school of medicine of the university of southern california,los angeles,ca, United States, department of pathogen biology and immunology,jiangsu key laboratory of pathogen biology,nanjing medical university,nanjing,jiangsu, China, department of pathogen biology and immunology,school of medicine,anhui university of science and technology,huainan,anhui, China, department of central laboratory,the affiliated people's hospital,jiangsu university,zhenjiang,jiangsu, China, department of pathogen biology and immunology,jiangsu key laboratory of pathogen biology,nanjing medical university,nanjing,jiangsu, China, department of central laboratory,the affiliated people's hospital,jiangsu university,zhenjiang,jiangsu, China, department of pathogen biology and immunology,jiangsu key laboratory of pathogen biology,nanjing medical university,nanjing,jiangsu, China
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|