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Responses of Murine and Human Macrophages to Leptospiral Infection: A Study Using Comparative Array Analysis
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نویسنده
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xue f. ,zhao x. ,yang y. ,zhao j. ,yang y. ,cao y. ,hong c. ,liu y. ,sun l. ,huang m. ,gu j.
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منبع
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plos neglected tropical diseases - 2013 - دوره : 7 - شماره : 10
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چکیده
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Leptospirosis is a re-emerging tropical infectious disease caused by pathogenic leptospira spp. the different host innate immune responses are partially related to the different severities of leptospirosis. in this study,we employed transcriptomics and cytokine arrays to comparatively calculate the responses of murine peritoneal macrophages (mpms) and human peripheral blood monocytes (hbms) to leptospiral infection. we uncovered a series of different expression profiles of these two immune cells. the percentages of regulated genes in several biological processes of mpms,such as antigen processing and presentation,membrane potential regulation,and the innate immune response,etc.,were much greater than those of hbms (>2-fold). in mpms and hbms,the caspase-8 and fas-associated protein with death domain (fadd)-like apoptosis regulator genes were significantly up-regulated,which supported previous results that the caspase-8 and caspase-3 pathways play an important role in macrophage apoptosis during leptospiral infection. in addition,the key component of the complement pathway,c3,was only up-regulated in mpms. furthermore,several cytokines,e.g. interleukin 10 (il-10) and tumor necrosis factor alpha (tnf-alpha),were differentially expressed at both mrna and protein levels in mpms and hbms. some of the differential expressions were proved to be pathogenic leptospira-specific regulations at mrna level or protein level. though it is still unclear why some animals are resistant and others are susceptible to leptospiral infection,this comparative study based on transcriptomics and cytokine arrays partially uncovered the differences of murine resistance and human susceptibility to leptospirosis. taken together,these findings will facilitate further molecular studies on the innate immune response to leptospiral infection. © 2013 xue et al.
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آدرس
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beijing friendship hospital,capital medical university,beijing,china,beijing tropical medicine research institute,beijing,china,beijing key laboratory for research on prevention and treatment of tropical diseases,beijing,china,beijing institute of biotechnology,beijing, China, beijing institute of biotechnology,beijing, China, division of parasitic vaccines,institute for biological product control,national institutes for food and drug control,beijing, China, school of life sciences,tsinghua university,beijing, China, department of neurobiology,capital medical university,beijing,china,beijing key laboratory of major brain disorders,beijing institute of brain disorders,beijing, China, college of veterinary medicine,jilin university,changchun, China, beijing friendship hospital,capital medical university,beijing, China, beijing friendship hospital,capital medical university,beijing, China, beijing friendship hospital,capital medical university,beijing,china,beijing tropical medicine research institute,beijing,china,beijing key laboratory for research on prevention and treatment of tropical diseases,beijing, China, beijing friendship hospital,capital medical university,beijing,china,beijing tropical medicine research institute,beijing,china,beijing key laboratory for research on prevention and treatment of tropical diseases,beijing, China, beijing friendship hospital,capital medical university,beijing,china,beijing tropical medicine research institute,beijing,china,beijing key laboratory for research on prevention and treatment of tropical diseases,beijing, China
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Authors
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