|
|
|
|
Bat Accelerated Regions Identify a Bat Forelimb Specific Enhancer in the HoxD Locus
|
|
|
|
|
|
|
|
نویسنده
|
booker b.m. ,friedrich t. ,mason m.k. ,vandermeer j.e. ,zhao j. ,eckalbar w.l. ,logan m. ,illing n. ,pollard k.s. ,ahituv n.
|
|
منبع
|
plos genetics - 2016 - دوره : 12 - شماره : 3
|
|
چکیده
|
The molecular events leading to the development of the bat wing remain largely unknown,and are thought to be caused,in part,by changes in gene expression during limb development. these expression changes could be instigated by variations in gene regulatory enhancers. here,we used a comparative genomics approach to identify regions that evolved rapidly in the bat ancestor,but are highly conserved in other vertebrates. we discovered 166 bat accelerated regions (bars) that overlap h3k27ac and p300 chip-seq peaks in developing mouse limbs. using a mouse enhancer assay,we show that five myotis lucifugus bars drive gene expression in the developing mouse limb,with the majority showing differential enhancer activity compared to the mouse orthologous bar sequences. these include bar116,which is located telomeric to the hoxd cluster and had robust forelimb expression for the m. lucifugus sequence and no activity for the mouse sequence at embryonic day 12.5. developing limb expression analysis of hoxd10-hoxd13 in miniopterus natalensis bats showed a high-forelimb weak-hindlimb expression for hoxd10-hoxd11,similar to the expression trend observed for m. lucifugus bar116 in mice,suggesting that it could be involved in the regulation of the bat hoxd complex. combined,our results highlight novel regulatory regions that could be instrumental for the morphological differences leading to the development of the bat wing. © 2016 booker et al.
|
|
|
|
|
آدرس
|
department of bioengineering and therapeutic sciences,university of california san francisco,san francisco,ca,united states,institute for human genetics,university of california san francisco,san francisco,ca, United States, institute for human genetics,university of california san francisco,san francisco,ca,united states,gladstone institutes,san francisco,ca, United States, department of molecular and cell biology,university of cape town,cape town, South Africa, department of bioengineering and therapeutic sciences,university of california san francisco,san francisco,ca,united states,institute for human genetics,university of california san francisco,san francisco,ca, United States, department of bioengineering and therapeutic sciences,university of california san francisco,san francisco,ca,united states,institute for human genetics,university of california san francisco,san francisco,ca,united states,key laboratory of advanced control and optimization for chemical processes of the ministry of education,east china university of science and technology,shanghai, China, department of bioengineering and therapeutic sciences,university of california san francisco,san francisco,ca,united states,institute for human genetics,university of california san francisco,san francisco,ca, United States, division of developmental biology,mrc-national institute for medical research,mill hill,london,united kingdom,randall division of cell and molecular biophysics,king’s college london,guys campus,london, United Kingdom, department of molecular and cell biology,university of cape town,cape town, South Africa, institute for human genetics,university of california san francisco,san francisco,ca,united states,gladstone institutes,san francisco,ca,united states,division of biostatistics,university of california san francisco,san francisco,ca, United States, department of bioengineering and therapeutic sciences,university of california san francisco,san francisco,ca,united states,institute for human genetics,university of california san francisco,san francisco,ca, United States
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|