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A Cohesin-Based Partitioning Mechanism Revealed upon Transcriptional Inactivation of Centromere
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نویسنده
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tsabar m. ,haase j. ,harrison b. ,snider c.e. ,eldridge b. ,kaminsky l. ,hine r.m. ,haber j.e. ,bloom k.
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منبع
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plos genetics - 2016 - دوره : 12 - شماره : 4
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چکیده
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Transcriptional inactivation of the budding yeast centromere has been a widely used tool in studies of chromosome segregation and aneuploidy. in haploid cells when an essential chromosome contains a single conditionally inactivated centromere (gal-cen),cell growth rate is slowed and segregation fidelity is reduced; but colony formation is nearly 100%. pedigree analysis revealed that only 30% of the time both mother and daughter cell inherit the gal-cen chromosome. the reduced segregation capacity of the gal-cen chromosome is further compromised upon reduction of pericentric cohesin (mcm21∆),as reflected in a further diminishment of the mif2 kinetochore protein at gal-cen. by redistributing cohesin from the nucleolus to the pericentromere (by deleting sir2),there is increased presence of the kinetochore protein mif2 at gal-cen and restoration of cell viability. these studies identify the ability of cohesin to promote chromosome segregation via kinetochore assembly,in a situation where the centromere has been severely compromised. © 2016 tsabar et al.
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آدرس
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department of biology and rosenstiel basic medical sciences research center,brandeis university,waltham,ma, United States, department of biology,university of north carolina at chapel hill,chapel hill,nc, United States, department of biology,university of north carolina at chapel hill,chapel hill,nc, United States, department of biology,university of north carolina at chapel hill,chapel hill,nc, United States, department of biology,university of north carolina at chapel hill,chapel hill,nc, United States, department of biology and rosenstiel basic medical sciences research center,brandeis university,waltham,ma, United States, department of biology and rosenstiel basic medical sciences research center,brandeis university,waltham,ma, United States, department of biology and rosenstiel basic medical sciences research center,brandeis university,waltham,ma, United States, department of biology,university of north carolina at chapel hill,chapel hill,nc, United States
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Authors
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