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RAB-10 Promotes EHBP-1 Bridging of Filamentous Actin and Tubular Recycling Endosomes
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نویسنده
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wang p. ,liu h. ,wang y. ,liu o. ,zhang j. ,gleason a. ,yang z. ,wang h. ,shi a. ,grant b.d.
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منبع
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plos genetics - 2016 - دوره : 12 - شماره : 6
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چکیده
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Ehbp-1 (ehbp1) is a conserved regulator of endocytic recycling,acting as an effector of small gtpases including rab-10 (rab10). here we present evidence that ehbp-1 associates with tubular endosomal phosphatidylinositol-4,5-bisphosphate [pi(4,5)p2] enriched membranes through an n-terminal c2-like (nt-c2) domain,and define residues within the nt-c2 domain that mediate membrane interaction. furthermore,our results indicate that the ehbp-1 central calponin homology (ch) domain binds to actin microfilaments in a reaction that is stimulated by rab-10(gtp). loss of any aspect of this rab-10/ehbp-1 system in the c. elegans intestinal epithelium leads to retention of basolateral recycling cargo in endosomes that have lost their normal tubular endosomal network (ten) organization. we propose a mechanism whereby rab-10 promotes the ability of endosome-bound ehbp-1 to also bind to the actin cytoskeleton,thereby promoting endosomal tubulation. © 2016 wang et al.
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آدرس
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department of medical genetics,school of basic medicine and the collaborative innovation center for brain science,tongji medical college,huazhong university of science and technology,wuhan,hubei, China, department of medical genetics,school of basic medicine and the collaborative innovation center for brain science,tongji medical college,huazhong university of science and technology,wuhan,hubei, China, department of medical genetics,school of basic medicine and the collaborative innovation center for brain science,tongji medical college,huazhong university of science and technology,wuhan,hubei, China, department of molecular biology and biochemistry,rutgers university,piscataway,nj, United States, department of medical genetics,school of basic medicine and the collaborative innovation center for brain science,tongji medical college,huazhong university of science and technology,wuhan,hubei, China, department of molecular biology and biochemistry,rutgers university,piscataway,nj, United States, department of medical genetics,school of basic medicine and the collaborative innovation center for brain science,tongji medical college,huazhong university of science and technology,wuhan,hubei, China, department of medical genetics,school of basic medicine and the collaborative innovation center for brain science,tongji medical college,huazhong university of science and technology,wuhan,hubei, China, department of medical genetics,school of basic medicine and the collaborative innovation center for brain science,tongji medical college,huazhong university of science and technology,wuhan,hubei,china,institute for brain research,huazhong university of science and technology,wuhan,hubei,china,key laboratory of neurological disease of national education ministry,tongji medical college,huazhong university of science and technology,wuhan,hubei, China, department of molecular biology and biochemistry,rutgers university,piscataway,nj, United States
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Authors
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