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Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis
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نویسنده
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li z. ,wang s. ,cheng j. ,su c. ,zhong s. ,liu q. ,fang y. ,yu y. ,lv h. ,zheng y. ,zheng b.
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منبع
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plos genetics - 2016 - دوره : 12 - شماره : 11
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چکیده
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Lariat rnas formed as by-products of splicing are quickly degraded by the rna debranching enzyme 1 (dbr1),leading to their turnover. null dbr1 mutants in both animals and plants are embryo lethal,but the mechanism underlying the lethality remains unclear. here we characterized a weak mutant allele of dbr1 in arabidopsis,dbr1-2,and showed that a global increase in lariat rnas was unexpectedly accompanied by a genome-wide reduction in mirna accumulation. the dbr1-2 mutation had no effects on expression of mirna biogenesis genes or primary mirnas (pri-mirnas),but the association of pri-mirnas with the dcl1/hyl1 dicing complex was impaired. lariat rnas were associated with the dcl1/hyl1 dicing complex in vivo and competitively inhibited the binding of hyl1 with pri-mirna. consistent with the impacts of lariat rnas on mirna biogenesis,over-expression of lariat rnas reduced mirna accumulation. lariat rnas localized in nuclear bodies,and partially co-localize with hyl1,and both dcl1 and hyl1 were mis-localized in dbr1-2. together with our findings that nearly four hundred lariat rnas exist in wild type plants and that these lariat rnas also associate with the dcl1/hyl1 dicing complex in vivo,we thus propose that lariat rnas,as decoys,inhibit mirna processing,suggesting a hitherto unknown layer of regulation in mirna biogenesis. © 2016 li et al.
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آدرس
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state key laboratory of genetic engineering,collaborative innovation center for genetics and development,school of life sciences,fudan university,shanghai, China, faculty of life science and technology,kunming university of science and technology,kunming, China, state key laboratory of genetic engineering,collaborative innovation center for genetics and development,school of life sciences,fudan university,shanghai, China, state key laboratory of genetic engineering,collaborative innovation center for genetics and development,school of life sciences,fudan university,shanghai, China, state key laboratory of genetic engineering,collaborative innovation center for genetics and development,school of life sciences,fudan university,shanghai, China, national key laboratory of plant molecular genetics,shanghai institute of plant physiology and ecology,chinese academy of sciences,shanghai, China, national key laboratory of plant molecular genetics,shanghai institute of plant physiology and ecology,chinese academy of sciences,shanghai, China, state key laboratory of genetic engineering,collaborative innovation center for genetics and development,school of life sciences,fudan university,shanghai, China, state key laboratory of genetic engineering,collaborative innovation center for genetics and development,school of life sciences,fudan university,shanghai, China, yunnan key laboratory of primate biomedical research,institute of primate translational medicine,kunming university of science and technology,kunming, China, state key laboratory of genetic engineering,collaborative innovation center for genetics and development,school of life sciences,fudan university,shanghai, China
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Authors
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