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MTV,an ssDNA Protecting Complex Essential for Transposon-Based Telomere Maintenance in Drosophila
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نویسنده
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zhang y. ,zhang l. ,tang x. ,bhardwaj s.r. ,ji j. ,rong y.s.
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منبع
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plos genetics - 2016 - دوره : 12 - شماره : 11
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چکیده
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Multiple complexes protect telomeres. in telomerase-maintained organisms,shelterin related complexes occupy the duplex region while the cst and tpp1-pot1 complexes bind the single stranded overhang of telomeres. drosophila uses a transposon-based mechanism for end protection. we showed that the hoap-hiphop complex occupies the duplex region. whether an ssdna-binding complex exists is not known. here we discover a novel protein,tea,that is specifically enriched at telomeres to prevent telomere fusion. we also identify a complex consisting of tea and two known capping proteins,ver and moi. the moi-tea-ver (mtv) complex purified in vitro binds and protects ssdna in a sequence-independent manner. tea recruits ver and moi to telomeres,and point mutations disrupting mtv interaction in vitro result in telomere uncapping,consistent with these proteins functioning as a complex in vivo. mtv thus shares functional similarities with cst or tpp1-pot1 in protecting ssdna,highlighting a conserved feature in end protecting mechanisms. © 2016,public library of science.
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آدرس
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state key laboratory of bio-control,institute of entomology,school of life sciences,sun yat-sen university,guangzhou,china,laboratory of biochemistry and molecular biology,national cancer institute,national institutes of health,bethesda,md, United States, laboratory of biochemistry and molecular biology,national cancer institute,national institutes of health,bethesda,md, United States, laboratory of biochemistry and molecular biology,national cancer institute,national institutes of health,bethesda,md, United States, laboratory of biochemistry and molecular biology,national cancer institute,national institutes of health,bethesda,md, United States, state key laboratory of bio-control,institute of entomology,school of life sciences,sun yat-sen university,guangzhou, China, state key laboratory of bio-control,institute of entomology,school of life sciences,sun yat-sen university,guangzhou,china,laboratory of biochemistry and molecular biology,national cancer institute,national institutes of health,bethesda,md, United States
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Authors
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