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TCP4-dependent induction of CONSTANS transcription requires GIGANTEA in photoperiodic flowering in Arabidopsis
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نویسنده
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kubota a. ,ito s. ,shim j.s. ,johnson r.s. ,song y.h. ,breton g. ,goralogia g.s. ,kwon m.s. ,laboy cintrón d. ,koyama t. ,ohme-takagi m. ,pruneda-paz j.l. ,kay s.a. ,maccoss m.j. ,imaizumi t.
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منبع
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plos genetics - 2017 - دوره : 13 - شماره : 6
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چکیده
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Photoperiod is one of the most reliable environmental cues for plants to regulate flowering timing. in arabidopsis thaliana,constans (co) transcription factor plays a central role in regulating photoperiodic flowering. in contrast to posttranslational regulation of co protein,still little was known about co transcriptional regulation. here we show that the cincinnata (cin) clade of class ii teosinte branched 1/ cycloidea/ proliferating cell nuclear antigen factor (tcp) proteins act as co activators. our yeast one-hybrid analysis revealed that class ii cin-tcps,including tcp4,bind to the co promoter. tcp4 induces co expression around dusk by directly associating with the co promoter in vivo. in addition,tcp4 binds to another flowering regulator,gigantea (gi),in the nucleus,and induces co expression in a gi-dependent manner. the physical association of tcp4 with the co promoter was reduced in the gi mutant,suggesting that gi may enhance the dna-binding ability of tcp4. our tandem affinity purification coupled with mass spectrometry (tap-ms) analysis identified all class ii cin-tcps as the components of the in vivo tcp4 complex,and the gi mutant did not alter the composition of the tcp4 complex. taken together,our results demonstrate a novel function of cin-tcps as photoperiodic flowering regulators,which may contribute to coordinating plant development with flowering regulation. © 2017 kubota et al.
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آدرس
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department of biology,university of washington,seattle,wa, United States, department of biology,university of washington,seattle,wa,united states,department of botany,graduate school of science,kyoto university,kyoto, Japan, department of biology,university of washington,seattle,wa, United States, department of genome sciences,university of washington,seattle,wa, United States, department of life sciences,ajou university,suwon, South Korea, section of cell and developmental biology,division of biological sciences,university of california at san diego,la jolla,ca,united states,department of integrative biology and pharmacology,mcgovern medical school,houston,tx, United States, department of biology,university of washington,seattle,wa, United States, department of biology,university of washington,seattle,wa, United States, department of biology,university of washington,seattle,wa, United States, bioorganic research center,suntory foundation for life sciences,kyoto, Japan, graduate school of science and engineering,saitama university,saitama, Japan, section of cell and developmental biology,division of biological sciences,university of california at san diego,la jolla,ca, United States, keck school of medicine,university of southern california,los angeles,ca, United States, department of genome sciences,university of washington,seattle,wa, United States, department of biology,university of washington,seattle,wa, United States
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Authors
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