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Overexpression of the WOX gene STENOFOLIA improves biomass yield and sugar release in transgenic grasses and display altered cytokinin homeostasis
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نویسنده
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wang h. ,niu l. ,fu c. ,meng y. ,sang d. ,yin p. ,wu j. ,tang y. ,lu t. ,wang z.-y. ,tadege m. ,lin h.
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منبع
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plos genetics - 2017 - دوره : 13 - شماره : 3
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چکیده
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Lignocellulosic biomass can be a significant source of renewable clean energy with continued improvement in biomass yield and bioconversion strategies. in higher plants,the leaf blade is the central energy convertor where solar energy and co2are assimilated to make the building blocks for biomass production. here we report that introducing the leaf blade development regulator stenofolia (stf),a wox family transcription factor,into the biofuel crop switchgrass,significantly improves both biomass yield and sugar release. we found that stf overexpressing switchgrass plants produced approximately 2-fold more dry biomass and release approximately 1.8-fold more solubilized sugars without pretreatment compared to controls. the biomass increase was attributed mainly to increased leaf width and stem thickness,which was also consistent in stf transgenic rice and brachypodium,and appeared to be caused by enhanced cell proliferation. stf directly binds to multiple regions in the promoters of some cytokinin oxidase/dehydrogenase (ckx) genes and represses their expression in all three transgenic grasses. this repression was accompanied by a significant increase in active cytokinin content in transgenic rice leaves,suggesting that the increase in biomass productivity and sugar release could at least in part be associated with improved cytokinin levels caused by repression of cytokinin degrading enzymes. our study provides a new tool for improving biomass feedstock yield in bioenergy crops,and uncovers a novel mechanistic insight in the function of stf,which may also apply to other repressive wox genes that are master regulators of several key plant developmental programs. © 2017 wang et al.
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آدرس
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biotechnology research institute,chinese academy of agricultural sciences,beijing, China, biotechnology research institute,chinese academy of agricultural sciences,beijing,china,department of plant and soil sciences,institute for agricultural biosciences,oklahoma state university,3210 sam noble parkway,ardmore,ok, United States, forage improvement division,samuel roberts noble foundation,2510 sam noble parkway,ardmore,ok,united states,qingdao institute of bioenergy and bioprocess technology,chinese academy of sciences,qingdao, China, biotechnology research institute,chinese academy of agricultural sciences,beijing, China, department of plant and soil sciences,institute for agricultural biosciences,oklahoma state university,3210 sam noble parkway,ardmore,ok,united states,institute for systems genetics,new york university,langone medical center,new york,ny, United States, biotechnology research institute,chinese academy of agricultural sciences,beijing, China, biotechnology research institute,chinese academy of agricultural sciences,beijing, China, plant biology division, United States, biotechnology research institute,chinese academy of agricultural sciences,beijing, China, forage improvement division,samuel roberts noble foundation,2510 sam noble parkway,ardmore,ok, United States, department of plant and soil sciences,institute for agricultural biosciences,oklahoma state university,3210 sam noble parkway,ardmore,ok, United States, biotechnology research institute,chinese academy of agricultural sciences,beijing,china,department of plant and soil sciences,institute for agricultural biosciences,oklahoma state university,3210 sam noble parkway,ardmore,ok, United States
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Authors
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