|
|
|
|
Xist RNA repeat E is essential for ASH2L recruitment to the inactive X and regulates histone modifications and escape gene expression
|
|
|
|
|
|
|
|
نویسنده
|
yue m. ,ogawa a. ,yamada n. ,charles richard j.l. ,barski a. ,ogawa y.
|
|
منبع
|
plos genetics - 2017 - دوره : 13 - شماره : 7
|
|
چکیده
|
Long non-coding rna xist plays a crucial role in establishing and maintaining x-chromosome inactivation (xci) which is a paradigm of long non-coding rna-mediated gene regulation. xist has xist-specific repeat elements a-f which are conserved among eutherian mammals,underscoring their functional importance. here we report that xist rna repeat e,a conserved xist repeat element in the xist exon 7,interacts with ash2l and contributes to maintenance of escape gene expression level on the inactive x-chromosome (xi) during xci. the xist repeat e-deletion mutant female es cells show the depletion of ash2l from the xi upon differentiation. furthermore,a subset of escape genes exhibits unexpectedly higher expression in the repeat e mutant cells than the cells expressing wildtype xist during x-inactivation,whereas the silencing of x-linked non-escape genes is not affected. we discuss the implications of these results to understand the role of ash2l and xist repeat e for histone modifications and escape gene regulation during random x-chromosome inactivation. © 2017 yue et al.
|
|
|
|
|
آدرس
|
division of reproductive sciences,perinatal institute,cincinnati children’s hospital medical center,cincinnati,oh,united states,department of pediatrics,university of cincinnati college of medicine,cincinnati,oh, United States, division of reproductive sciences,perinatal institute,cincinnati children’s hospital medical center,cincinnati,oh, United States, division of reproductive sciences,perinatal institute,cincinnati children’s hospital medical center,cincinnati,oh,united states,department of pediatrics,university of cincinnati college of medicine,cincinnati,oh, United States, division of reproductive sciences,perinatal institute,cincinnati children’s hospital medical center,cincinnati,oh,united states,department of pediatrics,university of cincinnati college of medicine,cincinnati,oh, United States, department of pediatrics,university of cincinnati college of medicine,cincinnati,oh,united states,division of allergy & immunology and human genetics,cincinnati children’s hospital medical center,cincinnati,oh, United States, division of reproductive sciences,perinatal institute,cincinnati children’s hospital medical center,cincinnati,oh,united states,department of pediatrics,university of cincinnati college of medicine,cincinnati,oh, United States
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|