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Analysis of the Phlebiopsis gigantea Genome,Transcriptome and Secretome Provides Insight into Its Pioneer Colonization Strategies of Wood
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نویسنده
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hori c. ,ishida t. ,igarashi k. ,samejima m. ,suzuki h. ,master e. ,ferreira p. ,ruiz-dueñas f.j. ,held b. ,canessa p. ,larrondo l.f. ,schmoll m. ,druzhinina i.s. ,kubicek c.p. ,gaskell j.a. ,kersten p. ,st. john f. ,glasner j. ,sabat g. ,splinter bondurant s. ,syed k. ,yadav j. ,mgbeahuruike a.c. ,kovalchuk a. ,asiegbu f.o. ,lackner g. ,hoffmeister d. ,rencoret j. ,gutiérrez a. ,sun h. ,lindquist e. ,barry k. ,riley r. ,grigoriev i.v. ,henrissat b. ,kües u. ,berka r.m. ,martínez a.t. ,covert s.f. ,blanchette r.a. ,cullen d.
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منبع
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plos genetics - 2014 - دوره : 10 - شماره : 12
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چکیده
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Collectively classified as white-rot fungi,certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. a small subset of these agaricomycetes,exemplified by phlebiopsis gigantea,is capable of colonizing freshly exposed conifer sapwood despite its high content of extractives,which retards the establishment of other fungal species. the mechanism(s) by which p. gigantea tolerates and metabolizes resinous compounds have not been explored. here,we report the annotated p. gigantea genome and compare profiles of its transcriptome and secretome when cultured on fresh-cut versus solvent-extracted loblolly pine wood. the p. gigantea genome contains a conventional repertoire of hydrolase genes involved in cellulose/hemicellulose degradation,whose patterns of expression were relatively unperturbed by the absence of extractives. the expression of genes typically ascribed to lignin degradation was also largely unaffected. in contrast,genes likely involved in the transformation and detoxification of wood extractives were highly induced in its presence. their products included an abc transporter,lipases,cytochrome p450s,glutathione s-transferase and aldehyde dehydrogenase. other regulated genes of unknown function and several constitutively expressed genes are also likely involved in p. gigantea's extractives metabolism. these results contribute to our fundamental understanding of pioneer colonization of conifer wood and provide insight into the diverse chemistries employed by fungi in carbon cycling processes. © 2014.
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آدرس
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department of biomaterials sciences,university of tokyo,tokyo, Japan, department of biomaterials sciences,university of tokyo,tokyo, Japan, department of biomaterials sciences,university of tokyo,tokyo, Japan, department of biomaterials sciences,university of tokyo,tokyo, Japan, department of chemical engineering,university of toronto,toronto,on, Canada, department of chemical engineering,university of toronto,toronto,on, Canada, department of biochemistry and molecular and cellular biology and institute of biocomputation and physics of complex systems,university of zaragoza,zaragoza, Spain, centro de investigaciones biológicas,consejo superior de investigaciones cientificas,madrid, Spain, department of plant pathology,university of minnesota,st. paul,mn, United States, millennium nucleus for fungal integrative and synthetic biology and departamento de genética molecular y microbiología,facultad de ciencias biológicas,pontificia universidad católica de chile,santiago, Chile, millennium nucleus for fungal integrative and synthetic biology and departamento de genética molecular y microbiología,facultad de ciencias biológicas,pontificia universidad católica de chile,santiago, Chile, health and environment departmentn institute of technology gmbh,tulin, Austria, austrian center of industrial biotechnology and institute of chemical engineering,vienna university of technology,vienna, Austria, austrian center of industrial biotechnology and institute of chemical engineering,vienna university of technology,vienna, Austria, usda,forest products laboratory,madison,wi, United States, usda,forest products laboratory,madison,wi, United States, usda,forest products laboratory,madison,wi, United States, university of wisconsin biotechnology center,madison,wi, United States, university of wisconsin biotechnology center,madison,wi, United States, university of wisconsin biotechnology center,madison,wi, United States, department of environmental health,university of cincinnati,cincinnati,oh, United States, department of environmental health,university of cincinnati,cincinnati,oh, United States, department of forest sciences,university of helsinki,helsinki, Finland, department of forest sciences,university of helsinki,helsinki, Finland, department of forest sciences,university of helsinki,helsinki, Finland, department of pharmaceutical biology at the hans-knöll-institute,friedrich-schiller-university,jena, Germany, department of pharmaceutical biology at the hans-knöll-institute,friedrich-schiller-university,jena, Germany, instituto de recursos naturales y agrobiologia de sevilla,csic,seville, Spain, instituto de recursos naturales y agrobiologia de sevilla,csic,seville, Spain, us department of energy joint genome institute,walnut creek,ca, United States, us department of energy joint genome institute,walnut creek,ca, United States, us department of energy joint genome institute,walnut creek,ca, United States, us department of energy joint genome institute,walnut creek,ca, United States, us department of energy joint genome institute,walnut creek,ca, United States, architecture et fonction des macromolécules biologiques,unité mixte de recherche 7257,aix-marseille université,centre national de la recherche scientifique,marseille, France, molecular wood biotechnology and technical mycology,büsgen-institute,georg-august university göttingen,göttingen, Germany, novozymes,inc,davis,ca, United States, centro de investigaciones biológicas,consejo superior de investigaciones cientificas,madrid, Spain, warnell school of forestry and natural resources,university of georgia,athens,ga, United States, department of plant pathology,university of minnesota,st. paul,mn, United States, usda,forest products laboratory,madison,wi, United States
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Authors
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