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A Truncated NLR Protein,TIR-NBS2,Is Required for Activated Defense Responses in the exo70B1 Mutant
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نویسنده
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zhao t. ,rui l. ,li j. ,nishimura m.t. ,vogel j.p. ,liu n. ,liu s. ,zhao y. ,dangl j.l. ,tang d.
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منبع
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plos genetics - 2015 - دوره : 11 - شماره : 1
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چکیده
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During exocytosis,the evolutionarily conserved exocyst complex tethers golgi-derived vesicles to the target plasma membrane,a critical function for secretory pathways. here we show that exo70b1 loss-of-function mutants express activated defense responses upon infection and express enhanced resistance to fungal,oomycete and bacterial pathogens. in a screen for mutants that suppress exo70b1 resistance,we identified nine alleles of tir-nbs2 (tn2),suggesting that loss-of-function of exo70b1 leads to activation of this nucleotide binding domain and leucine-rich repeat-containing (nlr)-like disease resistance protein. this nlr-like protein is atypical because it lacks the lrr domain common in typical nlr receptors. in addition,we show that tn2 interacts with exo70b1 in yeast and in planta. our study thus provides a link between the exocyst complex and the function of a ‘tir-nbs only’ immune receptor like protein. our data are consistent with a speculative model wherein pathogen effectors could evolve to target exo70b1 to manipulate plant secretion machinery. tn2 could monitor exo70b1 integrity as part of an immune receptor complex. © 2015,public library of science. all rights reserved.
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آدرس
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the state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing, China, the state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing,china,graduate school of chinese academy of sciences,beijing, China, the state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing, China, howard hughes medical institute and department of biology,university of north carolina,chapel hill,nc, United States, western regional research center,united states department of agriculture,agricultural research service,albany,ca, United States, the state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing,china,graduate school of chinese academy of sciences,beijing, China, the state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing,china,graduate school of chinese academy of sciences,beijing, China, the state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing,china,graduate school of chinese academy of sciences,beijing, China, howard hughes medical institute and department of biology,university of north carolina,chapel hill,nc, United States, the state key laboratory of plant cell and chromosome engineering,institute of genetics and developmental biology,chinese academy of sciences,beijing, China
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Authors
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