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Genetic Interaction Mapping Reveals a Role for the SWI/SNF Nucleosome Remodeler in Spliceosome Activation in Fission Yeast
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نویسنده
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patrick k.l. ,ryan c.j. ,xu j. ,lipp j.j. ,nissen k.e. ,roguev a. ,shales m. ,krogan n.j. ,guthrie c.
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منبع
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plos genetics - 2015 - دوره : 11 - شماره : 3
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چکیده
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Although numerous regulatory connections between pre-mrna splicing and chromatin have been demonstrated,the precise mechanisms by which chromatin factors influence spliceosome assembly and/or catalysis remain unclear. to probe the genetic network of pre-mrna splicing in the fission yeast schizosaccharomyces pombe,we constructed an epistatic mini-array profile (e-map) and discovered many new connections between chromatin and splicing. notably,the nucleosome remodeler swi/snf had strong genetic interactions with components of the u2 snrnp sf3 complex. overexpression of sf3 components in δswi/snf cells led to inefficient splicing of many fission yeast introns,predominantly those with non-consensus splice sites. deletion of swi/snf decreased recruitment of the splicing atpase prp2,suggesting that swi/snf promotes co-transcriptional spliceosome assembly prior to first step catalysis. importantly,defects in swi/snf as well as sf3 overexpression each altered nucleosome occupancy along intron-containing genes,illustrating that the chromatin landscape both affects—and is affected by—co-transcriptional splicing. © 2015 patrick et al.
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آدرس
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department of biochemistry and biophysics,university of california,san francisco,ca, United States, systems biology ireland,university college dublin,dublin,ireland,department of cellular and molecular pharmacology,university of california,san francisco,ca,united states,california institute for quantitative biosciences,qb3,san francisco,ca, United States, department of cellular and molecular pharmacology,university of california,san francisco,ca,united states,california institute for quantitative biosciences,qb3,san francisco,ca, United States, department of cellular and molecular pharmacology,university of california,san francisco,ca, United States, department of biochemistry and biophysics,university of california,san francisco,ca, United States, department of cellular and molecular pharmacology,university of california,san francisco,ca, United States, department of cellular and molecular pharmacology,university of california,san francisco,ca,united states,california institute for quantitative biosciences,qb3,san francisco,ca, United States, department of cellular and molecular pharmacology,university of california,san francisco,ca,united states,california institute for quantitative biosciences,qb3,san francisco,ca,united states,j. david gladstone institutes,san francisco,ca, United States, department of biochemistry and biophysics,university of california,san francisco,ca, United States
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Authors
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