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Distinct and Cooperative Activities of HESO1 and URT1 Nucleotidyl Transferases in MicroRNA Turnover in Arabidopsis
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نویسنده
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tu b. ,liu l. ,xu c. ,zhai j. ,li s. ,lopez m.a. ,zhao y. ,yu y. ,ramachandran v. ,ren g. ,yu b. ,li s. ,meyers b.c. ,mo b. ,chen x.
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منبع
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plos genetics - 2015 - دوره : 11 - شماره : 4
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چکیده
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3’ uridylation is increasingly recognized as a conserved rna modification process associated with rna turnover in eukaryotes. 2’-o-methylation on the 3’ terminal ribose protects micro(mi)rnas from 3’ truncation and 3’ uridylation in arabidopsis. previously,we identified heso1 as the nucleotidyl transferase that uridylates most unmethylated mirnas in vivo,but substantial 3’ tailing of mirnas still remains in heso1 loss-of-function mutants. in this study,we found that among nine other potential nucleotidyl transferases,utp:rna uridylyltransferase 1 (urt1) is the single most predominant nucleotidyl transferase that tails mirnas. urt1 and heso1 prefer substrates with different 3’ end nucleotides in vitro and act cooperatively to tail different forms of the same mirnas in vivo. moreover,both heso1 and urt1 exhibit nucleotidyl transferase activity on ago1-bound mirnas. although these enzymes are able to add long tails to ago1-bound mirnas,the tailed mirnas remain associated with ago1. moreover,tailing of ago1-bound mirna165/6 drastically reduces the slicing activity of ago1-mir165/6,suggesting that tailing reduces mirna activity. however,monouridylation of mir171a by urt1 endows the mirna the ability to trigger the biogenesis of secondary sirnas. therefore,3’ tailing could affect the activities of mirnas in addition to leading to mirna degradation. © 2015 tu et al.
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آدرس
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department of botany and plant sciences,institute of integrative genome biology,university of california,riverside,riverside,ca,united states,rice research institute,sichuan agricultural university,chengdu wenjiang,sichuan, China, department of botany and plant sciences,institute of integrative genome biology,university of california,riverside,riverside,ca,united states,shenzhen key laboratory of microbial genetic engineering,college of life sciences,shenzhen university,shenzhen, China, shenzhen key laboratory of microbial genetic engineering,college of life sciences,shenzhen university,shenzhen, China, department of plant & soil sciences,and delaware biotechnology institute,university of delaware,newark,de, United States, department of botany and plant sciences,institute of integrative genome biology,university of california,riverside,riverside,ca, United States, department of botany and plant sciences,institute of integrative genome biology,university of california,riverside,riverside,ca, United States, department of botany and plant sciences,institute of integrative genome biology,university of california,riverside,riverside,ca, United States, department of botany and plant sciences,institute of integrative genome biology,university of california,riverside,riverside,ca, United States, department of botany and plant sciences,institute of integrative genome biology,university of california,riverside,riverside,ca,united states,monsanto company,chesterfield,mo, United States, center for plant science innovation & school of biological sciences,university of nebraska-lincoln,lincoln,ne,united states,state key laboratory of genetic engineering,institute of plant biology and department of biochemistry,school of life sciences,fudan university,shanghai, China, center for plant science innovation & school of biological sciences,university of nebraska-lincoln,lincoln,ne, United States, rice research institute,sichuan agricultural university,chengdu wenjiang,sichuan, China, department of plant & soil sciences,and delaware biotechnology institute,university of delaware,newark,de, United States, shenzhen key laboratory of microbial genetic engineering,college of life sciences,shenzhen university,shenzhen, China, department of botany and plant sciences,institute of integrative genome biology,university of california,riverside,riverside,ca,united states,howard hughes medical institute,university of california,riverside,riverside,ca, United States
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Authors
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