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CCNYL1,but Not CCNY,Cooperates with CDK16 to Regulate Spermatogenesis in Mouse
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نویسنده
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zi z. ,zhang z. ,li q. ,an w. ,zeng l. ,gao d. ,yang y. ,zhu x. ,zeng r. ,shum w.w. ,wu j.
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منبع
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plos genetics - 2015 - دوره : 11 - شماره : 8
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چکیده
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Cyclin y-like 1 (ccnyl1) is a newly-identified member of the cyclin family and is highly similar in protein sequences to cyclin y (ccny). however,the function of ccnyl1 is poorly characterized in any organism. here we found that ccnyl1 was most abundantly expressed in the testis of mice and was about seven times higher than the level of ccny. male ccnyl1-/- mice were infertile,whereas both male and female ccny-/- mice displayed normal fertility. these results suggest that ccnyl1,but not ccny,is indispensable for male fertility. spermatozoa obtained from ccnyl1-/- mice displayed significantly impaired motility,and represented a thinned annulus region and/or a bent head. we found that the protein,but not the mrna,level of cyclin-dependent kinase 16 (cdk16) was decreased in the testis of ccnyl1-/- mice. further study demonstrated that ccnyl1 interacted with cdk16 and this interaction mutually increased the stability of these two proteins. moreover,the interaction increased the kinase activity of cdk16. in addition,we observed an alteration of phosphorylation levels of cdk16 in the presence of ccnyl1. we identified the phosphorylation sites of cdk16 by mass spectrometry and revealed that several phosphorylation modifications on the n-terminal region of cdk16 were indispensable for the ccnyl1 binding and the modulation of cdk16 kinase activity. our results therefore reveal a previously unrecognized role of ccnyl1 in regulating spermatogenesis through the interaction and modulation of cdk16. © 2015 zi et al.
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آدرس
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hefei national laboratory for physical sciences at microscale and school of life sciences,university of science & technology of china,hefei,anhui, China, key laboratory of systems biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai, China, key laboratory of systems biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai, China, key laboratory of systems biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai, China, state key laboratory of cell biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai, China, school of life science and technology,shanghaitech university,shanghai, China, key laboratory of systems biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai, China, state key laboratory of cell biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai, China, key laboratory of systems biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai,china,school of life science and technology,shanghaitech university,shanghai, China, school of life science and technology,shanghaitech university,shanghai, China, hefei national laboratory for physical sciences at microscale and school of life sciences,university of science & technology of china,hefei,anhui,china,key laboratory of systems biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai,china,school of life science and technology,shanghaitech university,shanghai,china,shanghai advanced research institute,chinese academy of sciences,shanghai, China
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Authors
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