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The Arabidopsis RCC1 Family Protein TCF1 Regulates Freezing Tolerance and Cold Acclimation through Modulating Lignin Biosynthesis
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نویسنده
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ji h. ,wang y. ,cloix c. ,li k. ,jenkins g.i. ,wang s. ,shang z. ,shi y. ,yang s. ,li x.
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منبع
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plos genetics - 2015 - دوره : 11 - شماره : 9
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چکیده
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Cell water permeability and cell wall properties are critical to survival of plant cells during freezing,however the underlying molecular mechanisms remain elusive. here,we report that a specifically cold-induced nuclear protein,tolerant to chilling and freezing 1 (tcf1),interacts with histones h3 and h4 and associates with chromatin containing a target gene,blue-copper-binding protein (bcb),encoding a glycosylphosphatidylinositol-anchored protein that regulates lignin biosynthesis. loss of tcf1 function leads to reduced bcb transcription through affecting h3k4me2 and h3k27me3 levels within the bcb gene,resulting in reduced lignin content and enhanced freezing tolerance. furthermore,plants with knocked-down bcb expression (amirna-bcb) under cold acclimation had reduced lignin accumulation and increased freezing tolerance. the pal1pal2 double mutant (lignin content reduced by 30% compared with wt) also showed the freezing tolerant phenotype,and tcf1 and bcb act upstream of pals to regulate lignin content. in addition,tcf1 acts independently of the cbf (c-repeat binding factor) pathway. our findings delineate a novel molecular pathway linking the tcf1-mediated cold-specific transcriptional program to lignin biosynthesis,thus achieving cell wall remodeling with increased freezing tolerance. © 2015 ji et al.
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آدرس
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state key laboratory of plant cell and chromosome engineering,center for agricultural research resources,institute of genetics and developmental biology,chinese academy of sciences,shijiazhuang,hebei,china,state key laboratory of agricultural microbiology,college of plant science and technology,huazhong agricultural university,wuhan, China, state key laboratory of plant cell and chromosome engineering,center for agricultural research resources,institute of genetics and developmental biology,chinese academy of sciences,shijiazhuang,hebei,china,state key laboratory of agricultural microbiology,college of plant science and technology,huazhong agricultural university,wuhan, China, institute of molecular cell and systems biology,college of medical,veterinary and life sciences,university of glasgow,bower building,glasgow, United Kingdom, state key laboratory of plant cell and chromosome engineering,center for agricultural research resources,institute of genetics and developmental biology,chinese academy of sciences,shijiazhuang,hebei,china,state key laboratory of agricultural microbiology,college of plant science and technology,huazhong agricultural university,wuhan, China, institute of molecular cell and systems biology,college of medical,veterinary and life sciences,university of glasgow,bower building,glasgow, United Kingdom, college of life science,hebei normal university,shijiazhuang, China, college of life science,hebei normal university,shijiazhuang, China, the state key laboratory of plant physiology and biochemistry,college of biological sciences agricultural university,beijing, China, the state key laboratory of plant physiology and biochemistry,college of biological sciences agricultural university,beijing, China, state key laboratory of plant cell and chromosome engineering,center for agricultural research resources,institute of genetics and developmental biology,chinese academy of sciences,shijiazhuang,hebei,china,state key laboratory of agricultural microbiology,college of plant science and technology,huazhong agricultural university,wuhan, China
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Authors
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