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Sequence-specific targeting of dosage compensation in Drosophila favors an active chromatin context
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نویسنده
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alekseyenko a.a. ,ho j.w.k. ,peng s. ,gelbart m. ,tolstorukov m.y. ,plachetka a. ,kharchenko p.v. ,jung y.l. ,gorchakov a.a. ,larschan e. ,gu t. ,minoda a. ,riddle n.c. ,schwartz y.b. ,elgin s.c.r. ,karpen g.h. ,pirrotta v. ,kuroda m.i. ,park p.j.
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منبع
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plos genetics - 2012 - دوره : 8 - شماره : 4
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چکیده
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The drosophila msl complex mediates dosage compensation by increasing transcription of the single x chromosome in males approximately two-fold. this is accomplished through recognition of the x chromosome and subsequent acetylation of histone h4k16 on x-linked genes. initial binding to the x is thought to occur at entry sites that contain a consensus sequence motif (msl recognition element or mre). however,this motif is only ~2 fold enriched on x,and only a fraction of the motifs on x are initially targeted. here we ask whether chromatin context could distinguish between utilized and non-utilized copies of the motif,by comparing their relative enrichment for histone modifications and chromosomal proteins mapped in the modencode project. through a comparative analysis of the chromatin features in male s2 cells (which contain msl complex) and female kc cells (which lack the complex),we find that the presence of active chromatin modifications,together with an elevated local gc content in the surrounding sequences,has strong predictive value for functional msl entry sites,independent of msl binding. we tested these sites for function in kc cells by rnai knockdown of sxl,resulting in induction of msl complex. we show that ectopic msl expression in kc cells leads to h4k16 acetylation around these sites and a relative increase in x chromosome transcription. collectively,our results support a model in which a pre-existing active chromatin environment,coincident with h3k36me3,contributes to msl entry site selection. the consequences of msl targeting of the male x chromosome include increase in nucleosome lability,enrichment for h4k16 acetylation and jil-1 kinase,and depletion of linker histone h1 on active x-linked genes. our analysis can serve as a model for identifying chromatin and local sequence features that may contribute to selection of functional protein binding sites in the genome. © 2012 alekseyenko et al.
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آدرس
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division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,department of genetics,harvard medical school,boston,ma, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,center for biomedical informatics,harvard medical school,boston,ma, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,center for biomedical informatics,harvard medical school,boston,ma, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,department of genetics,harvard medical school,boston,ma, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,center for biomedical informatics,harvard medical school,boston,ma, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,department of genetics,harvard medical school,boston,ma, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,center for biomedical informatics,harvard medical school,boston,ma, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,center for biomedical informatics,harvard medical school,boston,ma, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,department of genetics,harvard medical school,boston,ma, United States, department of molecular biology,cell biology,and biochemistry,brown university,providence,ri, United States, department of biology,washington university in st. louis,st. louis,mo, United States, department of molecular and cell biology,university of california berkeley,berkeley,ca,united states,department of genome dynamics,lawrence berkeley national lab,berkeley,ca, United States, department of biology,washington university in st. louis,st. louis,mo, United States, department of molecular biology,umeå university,umeå, Sweden, department of biology,washington university in st. louis,st. louis,mo, United States, department of molecular and cell biology,university of california berkeley,berkeley,ca, United States, department of molecular biology and biochemistry,rutgers university,piscataway,nj, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,department of genetics,harvard medical school,boston,ma, United States, division of genetics,department of medicine,brigham and women's hospital,harvard medical school,boston,ma,united states,center for biomedical informatics,harvard medical school,boston,ma, United States
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Authors
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