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Prdm5 regulates collagen gene transcription by association with RNA polymerase II in developing bone
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نویسنده
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galli g.g. ,honnens de lichtenberg k. ,carrara m. ,hans w. ,wuelling m. ,mentz b. ,multhaupt h.a. ,fog c.k. ,jensen k.t. ,rappsilber j. ,vortkamp a. ,coulton l. ,fuchs h. ,gailus-durner v. ,hrabě de angelis m. ,calogero r.a. ,couchman j.r. ,lund a.h.
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منبع
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plos genetics - 2012 - دوره : 8 - شماره : 5
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چکیده
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Prdm family members are transcriptional regulators involved in tissue specific differentiation. prdm5 has been reported to predominantly repress transcription,but a characterization of its molecular functions in a relevant biological context is lacking. we demonstrate here that prdm5 is highly expressed in developing bones; and,by genome-wide mapping of prdm5 occupancy in pre-osteoblastic cells,we uncover a novel and unique role for prdm5 in targeting all mouse collagen genes as well as several slrp proteoglycan genes. in particular,we show that prdm5 controls both collagen i transcription and fibrillogenesis by binding inside the col1a1 gene body and maintaining rna polymerase ii occupancy. in vivo,prdm5 loss results in delayed ossification involving a pronounced impairment in the assembly of fibrillar collagens. collectively,our results define a novel role for prdm5 in sustaining the transcriptional program necessary to the proper assembly of osteoblastic extracellular matrix. © 2012 galli et al.
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آدرس
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biotech research and innovation centre and centre for epigenetics,university of copenhagen,copenhagen, Denmark, biotech research and innovation centre and centre for epigenetics,university of copenhagen,copenhagen, Denmark, molecular biotechnology center,department of clinical and biological sciences,university of torino,torino, Italy, german mouse clinic,institute of experimental genetics,helmholtz zentrum münchen,german research center for environmental health gmbh,neuherberg, Germany, department of developmental biology,center for medical biotechnology,university duisburg-essen,essen, Germany, biotech research and innovation centre and centre for epigenetics,university of copenhagen,copenhagen, Denmark, department of biomedical sciences and bric,university of copenhagen,copenhagen, Denmark, biotech research and innovation centre and centre for epigenetics,university of copenhagen,copenhagen, Denmark, biotech research and innovation centre and centre for epigenetics,university of copenhagen,copenhagen, Denmark, wellcome trust centre for cell biology,university of edinburgh,edinburgh, United Kingdom, department of developmental biology,center for medical biotechnology,university duisburg-essen,essen, Germany, academic unit of bone biology,university of sheffield medical school,sheffield, United Kingdom, department of developmental biology,center for medical biotechnology,university duisburg-essen,essen, Germany, german mouse clinic,institute of experimental genetics,helmholtz zentrum münchen,german research center for environmental health gmbh,neuherberg, Germany, german mouse clinic,institute of experimental genetics,helmholtz zentrum münchen,german research center for environmental health gmbh,neuherberg,germany,chair of experimental genetics tum,freising-weihenstephan, Germany, molecular biotechnology center,department of clinical and biological sciences,university of torino,torino, Italy, department of biomedical sciences and bric,university of copenhagen,copenhagen, Denmark, biotech research and innovation centre and centre for epigenetics,university of copenhagen,copenhagen, Denmark
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Authors
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