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The CCR4-NOT complex is implicated in the viability of aneuploid yeasts
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نویسنده
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tange y. ,kurabayashi a. ,goto b. ,hoe k.-l. ,kim d.-u. ,park h.-o. ,hayles j. ,chikashige y. ,tsutumi c. ,hiraoka y. ,yamao f. ,nurse p. ,niwa o.
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منبع
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plos genetics - 2012 - دوره : 8 - شماره : 6
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چکیده
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To identify the genes required to sustain aneuploid viability,we screened a deletion library of non-essential genes in the fission yeast schizosaccharomyces pombe,in which most types of aneuploidy are eventually lethal to the cell. aneuploids remain viable for a period of time and can form colonies by reducing the extent of the aneuploidy. we hypothesized that a reduction in colony formation efficiency could be used to screen for gene deletions that compromise aneuploid viability. deletion mutants were used to measure the effects on the viability of spores derived from triploid meiosis and from a chromosome instability mutant. we found that the ccr4-not complex,an evolutionarily conserved general regulator of mrna turnover,and other related factors,including poly(a)-specific nuclease for mrna decay,are involved in aneuploid viability. defective mutations in ccr4-not complex components in the distantly related yeast saccharomyces cerevisiae also affected the viability of spores produced from triploid cells,suggesting that this complex has a conserved role in aneuploids. in addition,our findings suggest that the genes required for homologous recombination repair are important for aneuploid viability. © 2012 tange et al.
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آدرس
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kazusa dna research institute,kisarazu,chiba,japan,graduate school of frontier biosciences,osaka university,suita,osaka, Japan, kazusa dna research institute,kisarazu,chiba, Japan, kazusa dna research institute,kisarazu,chiba, Japan, chungnam national university,graduate school of new drug discovery and development,yusong-gu,daejeon, South Korea, aging research center,korea research institute of bioscience and biotechnology (kribb),yusong-gu,daejeon, South Korea, bioneer corporation,daejeon, South Korea, cancer research uk,the london research institute,london, United Kingdom, national institute of information and communications technology,kobe, Japan, national institute of information and communications technology,kobe, Japan, graduate school of frontier biosciences,osaka university,suita,osaka,japan,national institute of information and communications technology,kobe, Japan, national institute of genetics,mishima,shizuoka, Japan, cancer research uk,the london research institute,london,united kingdom,the rockefeller university,new york,ny, United States, kazusa dna research institute,kisarazu,chiba,japan,the rockefeller university,new york,ny, United States
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Authors
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