|
|
|
|
CED-10/Rac1 regulates endocytic recycling through the RAB-5 GAP TBC-2
|
|
|
|
|
|
|
|
نویسنده
|
sun l. ,liu o. ,desai j. ,karbassi f. ,sylvain m.-a. ,shi a. ,zhou z. ,rocheleau c.e. ,grant b.d.
|
|
منبع
|
plos genetics - 2012 - دوره : 8 - شماره : 7
|
|
چکیده
|
Rac1 is a founding member of the rho-gtpase family and a key regulator of membrane remodeling. in the context of apoptotic cell corpse engulfment,ced-10/rac1 acts with its bipartite guanine nucleotide exchange factor,ced-5/dock180-ced-12/elmo,in an evolutionarily conserved pathway to promote phagocytosis. here we show that in the context of the caenorhabditis elegans intestinal epithelium ced-10/rac1,ced-5/dock180,and ced-12/elmo promote basolateral recycling. furthermore,we show that ced-10 binds to the rab-5 gtpase activating protein tbc-2,that ced-10 contributes to recruitment of tbc-2 to endosomes,and that recycling cargo is trapped in recycling endosomes in ced-12,ced-10,and tbc-2 mutants. expression of gtpase defective rab-5(q78l) also traps recycling cargo. our results indicate that down-regulation of early endosome regulator rab-5/rab5 by a ced-5,ced-12,ced-10,tbc-2 cascade is an important step in the transport of cargo through the basolateral recycling endosome for delivery to the plasma membrane. © 2012 sun et al.
|
|
|
|
|
آدرس
|
department of molecular biology and biochemistry,rutgers university,piscataway,nj,united states,department of physiology and biophysics,boston university school of medicine,boston,ma, United States, department of molecular biology and biochemistry,rutgers university,piscataway,nj, United States, department of molecular biology and biochemistry,rutgers university,piscataway,nj, United States, division of endocrinology and metabolism,department of medicine,mcgill university and mcgill university health centre research institute,montreal,qc, Canada, division of endocrinology and metabolism,department of medicine,mcgill university and mcgill university health centre research institute,montreal,qc, Canada, department of molecular biology and biochemistry,rutgers university,piscataway,nj,united states,department of biology,stanford university,stanford,ca, United States, department of biochemistry and molecular biology,baylor college of medicine,houston,tx, United States, division of endocrinology and metabolism,department of medicine,mcgill university and mcgill university health centre research institute,montreal,qc, Canada, department of molecular biology and biochemistry,rutgers university,piscataway,nj, United States
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|