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Histone H2A mono-ubiquitination is a crucial step to mediate PRC1-dependent repression of developmental genes to maintain ES cell identity
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نویسنده
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endoh m. ,endo t.a. ,endoh t. ,isono k.-i. ,sharif j. ,ohara o. ,toyoda t. ,ito t. ,eskeland r. ,bickmore w.a. ,vidal m. ,bernstein b.e. ,koseki h.
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منبع
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plos genetics - 2012 - دوره : 8 - شماره : 7
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چکیده
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Two distinct polycomb complexes,prc1 and prc2,collaborate to maintain epigenetic repression of key developmental loci in embryonic stem cells (escs). prc1 and prc2 have histone modifying activities,catalyzing mono-ubiquitination of histone h2a (h2ak119u1) and trimethylation of h3 lysine 27 (h3k27me3),respectively. compared to h3k27me3,localization and the role of h2ak119u1 are not fully understood in escs. here we present genome-wide h2ak119u1 maps in escs and identify a group of genes at which h2ak119u1 is deposited in a ring1-dependent manner. these genes are a distinctive subset of genes with h3k27me3 enrichment and are the central targets of polycomb silencing that are required to maintain esc identity. we further show that the h2a ubiquitination activity of prc1 is dispensable for its target binding and its activity to compact chromatin at hox loci,but is indispensable for efficient repression of target genes and thereby esc maintenance. these data demonstrate that multiple effector mechanisms including h2a ubiquitination and chromatin compaction combine to mediate prc1-dependent repression of genes that are crucial for the maintenance of esc identity. utilization of these diverse effector mechanisms might provide a means to maintain a repressive state that is robust yet highly responsive to developmental cues during es cell self-renewal and differentiation. © 2012 endoh et al.
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آدرس
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laboratory for developmental genetics,riken research center for allergy and immunology,yokohama,japan,core research for evolutional science and technology,japan science and technology agency,yokohama, Japan, riken bioinformatics and system engineering division,yokohama, Japan, laboratory for developmental genetics,riken research center for allergy and immunology,yokohama, Japan, laboratory for developmental genetics,riken research center for allergy and immunology,yokohama, Japan, laboratory for developmental genetics,riken research center for allergy and immunology,yokohama, Japan, laboratories for immunogenomics,riken research center for allergy and immunology,yokohama, Japan, riken bioinformatics and system engineering division,yokohama, Japan, department of biochemistry,nagasaki university school of medicine,nagasaki, Japan, mrc human genetics unit,institute of genetics and molecular medicine,university of edinburgh,edinburgh, United Kingdom, mrc human genetics unit,institute of genetics and molecular medicine,university of edinburgh,edinburgh, United Kingdom, cell proliferation and development,centro de investigaciones biologicas,consejo superior de investigaciones científicas,madrid,spain,research unit for immunoepigenetics,riken research center for allergy and immunology,yokohama, Japan, molecular pathology unit and center for cancer research,massachusetts general hospital,charlestown,ma,united states,department of pathology,harvard medical school,boston,ma,united states,broad institute of harvard and mit,cambridge,ma, United States, laboratory for developmental genetics,riken research center for allergy and immunology,yokohama,japan,core research for evolutional science and technology,japan science and technology agency,yokohama, Japan
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Authors
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