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RsFA (YbeB) proteins are conserved ribosomal silencing factors
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نویسنده
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häuser r. ,pech m. ,kijek j. ,yamamoto h. ,titz b. ,naeve f. ,tovchigrechko a. ,yamamoto k. ,szaflarski w. ,takeuchi n. ,stellberger t. ,diefenbacher m.e. ,nierhaus k.h. ,uetz p.
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منبع
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plos genetics - 2012 - دوره : 8 - شماره : 7
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چکیده
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The ybeb (duf143) family of uncharacterized proteins is encoded by almost all bacterial and eukaryotic genomes but not archaea. while they have been shown to be associated with ribosomes,their molecular function remains unclear. here we show that ybeb is a ribosomal silencing factor (rsfa) in the stationary growth phase and during the transition from rich to poor media. a knock-out of the rsfa gene shows two strong phenotypes: (i) the viability of the mutant cells are sharply impaired during stationary phase (as shown by viability competition assays),and (ii) during transition from rich to poor media the mutant cells adapt slowly and show a growth block of more than 10 hours (as shown by growth competition assays). rsfa silences translation by binding to the l14 protein of the large ribosomal subunit and,as a consequence,impairs subunit joining (as shown by molecular modeling,reporter gene analysis,in vitro translation assays,and sucrose gradient analysis). this particular interaction is conserved in all species tested,including escherichia coli,treponema pallidum,streptococcus pneumoniae,synechocystis pcc 6803,as well as human mitochondria and maize chloroplasts (as demonstrated by yeast two-hybrid tests,pull-downs,and mutagenesis). rsfa is unrelated to the eukaryotic ribosomal anti-association/60s-assembly factor eif6,which also binds to l14,and is the first such factor in bacteria and organelles. rsfa helps cells to adapt to slow-growth/stationary phase conditions by down-regulating protein synthesis,one of the most energy-consuming processes in both bacterial and eukaryotic cells. © 2012 häuser et al.
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آدرس
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institute of toxicology and genetics,karlsruhe institute of technology (kit),karlsruhe,germany,german cancer research center (dkfz),heidelberg, Germany, abteilung vingron,ag ribosomen max-planck-institut für molekulare genetik,berlin,germany,institut für medizinische physik und biophysik,charité-universitätsmedizin berlin,berlin, Germany, abteilung vingron,ag ribosomen max-planck-institut für molekulare genetik,berlin,germany,institut für medizinische physik und biophysik,charité-universitätsmedizin berlin,berlin, Germany, abteilung vingron,ag ribosomen max-planck-institut für molekulare genetik,berlin,germany,institut für medizinische physik und biophysik,charité-universitätsmedizin berlin,berlin, Germany, institute of toxicology and genetics,karlsruhe institute of technology (kit),karlsruhe,germany,crump institute for molecular imaging,department of molecular and medical pharmacology,university of california los angeles,los angeles,ca, United States, institute of toxicology and genetics,karlsruhe institute of technology (kit),karlsruhe, Germany, j. craig venter institute (jcvi),rockville,md, United States, abteilung vingron,ag ribosomen max-planck-institut für molekulare genetik,berlin,germany,institut für medizinische physik und biophysik,charité-universitätsmedizin berlin,berlin, Germany, abteilung vingron,ag ribosomen max-planck-institut für molekulare genetik,berlin,germany,department of histology and embryology,poznan university of medical sciences,poznan, Poland, department of medical genome sciences,graduate school of frontier sciences,university of tokyo,kashiwa-shi,chiba, Japan, institute of toxicology and genetics,karlsruhe institute of technology (kit),karlsruhe, Germany, institute of toxicology and genetics,karlsruhe institute of technology (kit),karlsruhe, Germany, abteilung vingron,ag ribosomen max-planck-institut für molekulare genetik,berlin,germany,institut für medizinische physik und biophysik,charité-universitätsmedizin berlin,berlin, Germany, institute of toxicology and genetics,karlsruhe institute of technology (kit),karlsruhe,germany,proteros biostructures,martinsried,germany,center for the study of biological complexity,virginia commonwealth university,richmond,va, United States
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Authors
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