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The chicken frizzle feather is due to an α-Keratin (KRT75) mutation that causes a defective rachis
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نویسنده
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ng c.s. ,wu p. ,foley j. ,foley a. ,mcdonald m.-l. ,juan w.-t. ,huang c.-j. ,lai y.-t. ,lo w.-s. ,chen c.-f. ,leal s.m. ,zhang h. ,widelitz r.b. ,patel p.i. ,li w.-h. ,chuong c.-m.
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منبع
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plos genetics - 2012 - دوره : 8 - شماره : 7
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چکیده
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Feathers have complex forms and are an excellent model to study the development and evolution of morphologies. existing chicken feather mutants are especially useful for identifying genetic determinants of feather formation. this study focused on the gene f,underlying the frizzle feather trait that has a characteristic curled feather rachis and barbs in domestic chickens. our developmental biology studies identified defects in feather medulla formation,and physical studies revealed that the frizzle feather curls in a stepwise manner. the frizzle gene is transmitted in an autosomal incomplete dominant mode. a whole-genome linkage scan of five pedigrees with 2678 snps revealed association of the frizzle locus with a keratin gene-enriched region within the linkage group e22c19w28_e50c23. sequence analyses of the keratin gene cluster identified a 69 bp in-frame deletion in a conserved region of krt75,an α-keratin gene. retroviral-mediated expression of the mutated f cdna in the wild-type rectrix qualitatively changed the bending of the rachis with some features of frizzle feathers including irregular kinks,severe bending near their distal ends,and substantially higher variations among samples in comparison to normal feathers. these results confirmed krt75 as the f gene. this study demonstrates the potential of our approach for identifying genetic determinants of feather forms. © 2012 ng et al.
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آدرس
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biodiversity research center,academia sinica,taipei, Taiwan, department of pathology,keck school of medicine,university of southern california,los angeles,ca, United States, department of anatomy and cell biology,indiana university school of medicine,bloomington,in,united states,department of dermatology,indiana university school of medicine,bloomington,in, United States, department of anatomy and cell biology,indiana university school of medicine,bloomington,in,united states,department of dermatology,indiana university school of medicine,bloomington,in, United States, department of molecular and human genetics,baylor college of medicine,houston,tx, United States, institute of physics,academia sinica,taipei, Taiwan, biodiversity research center,academia sinica,taipei,taiwan,taiwan international graduate program,academia sinica,taipei,taiwan,graduate institute of biotechnology,national chung hsing university,taichung, Taiwan, biodiversity research center,academia sinica,taipei, Taiwan, biodiversity research center,academia sinica,taipei, Taiwan, department of animal sciences,national chung hsing university,taichung, Taiwan, department of dermatology,indiana university school of medicine,bloomington,in, United States, avian disease and oncology laboratory,agriculture research service,united states department of agriculture,east lansing,mi, United States, department of pathology,keck school of medicine,university of southern california,los angeles,ca, United States, institute for genetic medicine and center for craniofacial molecular biology,university of southern california,los angeles,ca, United States, biodiversity research center,academia sinica,taipei,taiwan,department of ecology and evolution,university of chicago,chicago,il, United States, department of pathology,keck school of medicine,university of southern california,los angeles,ca,united states,research center for developmental biology and regenerative medicine,national taiwan university,taipei, Taiwan
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Authors
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