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H4K20me1 Contributes to Downregulation of X-Linked Genes for C. elegans Dosage Compensation
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نویسنده
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vielle a. ,lang j. ,dong y. ,ercan s. ,kotwaliwale c. ,rechtsteiner a. ,appert a. ,chen q.b. ,dose a. ,egelhofer t. ,kimura h. ,stempor p. ,dernburg a. ,lieb j.d. ,strome s. ,ahringer j.
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منبع
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plos genetics - 2012 - دوره : 8 - شماره : 9
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چکیده
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The caenorhabditis elegans dosage compensation complex (dcc) equalizes x-chromosome gene dosage between xo males and xx hermaphrodites by two-fold repression of x-linked gene expression in hermaphrodites. the dcc localizes to the x chromosomes in hermaphrodites but not in males,and some subunits form a complex homologous to condensin. the mechanism by which the dcc downregulates gene expression remains unclear. here we show that the dcc controls the methylation state of lysine 20 of histone h4,leading to higher h4k20me1 and lower h4k20me3 levels on the x chromosomes of xx hermaphrodites relative to autosomes. we identify the pr-set7 ortholog set-1 and the suv4-20 ortholog set-4 as the major histone methyltransferases for monomethylation and di/trimethylation of h4k20,respectively,and provide evidence that x-chromosome enrichment of h4k20me1 involves inhibition of set-4 activity on the x. rnai knockdown of set-1 results in synthetic lethality with dosage compensation mutants and upregulation of x-linked gene expression,supporting a model whereby h4k20me1 functions with the condensin-like c. elegans dcc to repress transcription of x-linked genes. h4k20me1 is important for mitotic chromosome condensation in mammals,suggesting that increased h4k20me1 on the x may restrict access of the transcription machinery to x-linked genes via chromatin compaction. © 2012 vielle et al.
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آدرس
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the gurdon institute and department of genetics,university of cambridge,cambridge, United Kingdom, department of molecular,cell,and developmental biology,university of california santa cruz,santa cruz,ca, United States, the gurdon institute and department of genetics,university of cambridge,cambridge, United Kingdom, department of biology,carolina center for the genome sciences,lineberger comprehensive cancer center,the university of north carolina at chapel hill,chapel hill,nc,united states,center for genomics and systems biology,new york university,new york,ny, United States, department of molecular and cell biology and california institute for quantitative biosciences (qb3),university of california berkeley,berkeley,ca,united states,howard hughes medical institute,chevy chase,md, United States, department of molecular,cell,and developmental biology,university of california santa cruz,santa cruz,ca, United States, the gurdon institute and department of genetics,university of cambridge,cambridge, United Kingdom, department of biology,carolina center for the genome sciences,lineberger comprehensive cancer center,the university of north carolina at chapel hill,chapel hill,nc, United States, department of molecular and cell biology and california institute for quantitative biosciences (qb3),university of california berkeley,berkeley,ca,united states,howard hughes medical institute,chevy chase,md, United States, department of molecular,cell,and developmental biology,university of california santa cruz,santa cruz,ca, United States, graduate school for frontier biosciences,osaka university,osaka, Japan, the gurdon institute and department of genetics,university of cambridge,cambridge, United Kingdom, department of molecular and cell biology and california institute for quantitative biosciences (qb3),university of california berkeley,berkeley,ca,united states,howard hughes medical institute,chevy chase,md, United States, department of biology,carolina center for the genome sciences,lineberger comprehensive cancer center,the university of north carolina at chapel hill,chapel hill,nc, United States, department of molecular,cell,and developmental biology,university of california santa cruz,santa cruz,ca, United States, the gurdon institute and department of genetics,university of cambridge,cambridge, United Kingdom
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Authors
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