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DNA-Methylome Analysis of Mouse Intestinal Adenoma Identifies a Tumour-Specific Signature That Is Partly Conserved in Human Colon Cancer
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نویسنده
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grimm c. ,chavez l. ,vilardell m. ,farrall a.l. ,tierling s. ,böhm j.w. ,grote p. ,lienhard m. ,dietrich j. ,timmermann b. ,walter j. ,schweiger m.r. ,lehrach h. ,herwig r. ,herrmann b.g. ,morkel m.
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منبع
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plos genetics - 2013 - دوره : 9 - شماره : 2
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چکیده
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Aberrant cpg methylation is a universal epigenetic trait of cancer cell genomes. however,human cancer samples or cell lines preclude the investigation of epigenetic changes occurring early during tumour development. here,we have used medip-seq to analyse the dna methylome of apcmin adenoma as a model for intestinal cancer initiation,and we present a list of more than 13,000 recurring differentially methylated regions (dmrs) characterizing intestinal adenoma of the mouse. we show that polycomb repressive complex (prc) targets are strongly enriched among hypermethylated dmrs,and several prc2 components and dna methyltransferases were up-regulated in adenoma. we further demonstrate by bisulfite pyrosequencing of purified cell populations that the dmr signature arises de novo in adenoma cells rather than by expansion of a pre-existing pattern in intestinal stem cells or undifferentiated crypt cells. we found that epigenetic silencing of tumour suppressors,which occurs frequently in colon cancer,was rare in adenoma. quite strikingly,we identified a core set of dmrs,which is conserved between mouse adenoma and human colon cancer,thus possibly revealing a global panel of epigenetically modified genes for intestinal tumours. our data allow a distinction between early conserved epigenetic alterations occurring in intestinal adenoma and late stochastic events promoting colon cancer progression,and may facilitate the selection of more specific clinical epigenetic biomarkers. © 2013 grimm et al.
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آدرس
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max planck institute for molecular genetics,department of vertebrate genomics,berlin,germany,charité universitätsmedizin berlin,department of rheumatology,berlin, Germany, max planck institute for molecular genetics,department of vertebrate genomics,berlin, Germany, max planck institute for molecular genetics,department of vertebrate genomics,berlin, Germany, max planck institute for molecular genetics,department of developmental genetics,berlin, Germany, universität des saarlandes,fr 8.3 biowissenschaften,genetik/epigenetik campus,saarbrücken, Germany, universität des saarlandes,fr 8.3 biowissenschaften,genetik/epigenetik campus,saarbrücken, Germany, max planck institute for molecular genetics,department of developmental genetics,berlin, Germany, max planck institute for molecular genetics,department of vertebrate genomics,berlin, Germany, max planck institute for molecular genetics,department of vertebrate genomics,berlin, Germany, max planck institute for molecular genetics,next generation sequencing core facility,berlin, Germany, universität des saarlandes,fr 8.3 biowissenschaften,genetik/epigenetik campus,saarbrücken, Germany, max planck institute for molecular genetics,department of vertebrate genomics,berlin, Germany, max planck institute for molecular genetics,department of vertebrate genomics,berlin, Germany, max planck institute for molecular genetics,department of vertebrate genomics,berlin, Germany, max planck institute for molecular genetics,department of developmental genetics,berlin,germany,charité universitätsmedizin berlin,institute for medical genetics,berlin, Germany, max planck institute for molecular genetics,department of developmental genetics,berlin,germany,charité universitätsmedizin berlin,laboratory of molecular tumor pathology,berlin, Germany
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Authors
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