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Functional Genomic Analysis of the let-7 Regulatory Network in Caenorhabditis elegans
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نویسنده
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hunter s.e. ,finnegan e.f. ,zisoulis d.g. ,lovci m.t. ,melnik-martinez k.v. ,yeo g.w. ,pasquinelli a.e.
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منبع
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plos genetics - 2013 - دوره : 9 - شماره : 3
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چکیده
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The let-7 microrna (mirna) regulates cellular differentiation across many animal species. loss of let-7 activity causes abnormal development in caenorhabditis elegans and unchecked cellular proliferation in human cells,which contributes to tumorigenesis. these defects are due to improper expression of protein-coding genes normally under let-7 regulation. while some direct targets of let-7 have been identified,the genome-wide effect of let-7 insufficiency in a developing animal has not been fully investigated. here we report the results of molecular and genetic assays aimed at determining the global network of genes regulated by let-7 in c. elegans. by screening for mis-regulated genes that also contribute to let-7 mutant phenotypes,we derived a list of physiologically relevant potential targets of let-7 regulation. twenty new suppressors of the rupturing vulva or extra seam cell division phenotypes characteristic of let-7 mutants emerged. three of these genes,opt-2,prmt-1,and t27d12.1,were found to associate with argonaute in a let-7-dependent manner and are likely novel direct targets of this mirna. overall,a complex network of genes with various activities is subject to let-7 regulation to coordinate developmental timing across tissues during worm development. © 2013 hunter et al.
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آدرس
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division of biology,university of california san diego,la jolla,ca, United States, division of biology,university of california san diego,la jolla,ca, United States, division of biology,university of california san diego,la jolla,ca, United States, department of cellular and molecular medicine,university of california san diego,la jolla,ca,united states,stem cell program,university of california san diego,la jolla,ca,united states,institute for genomic medicine,university of california san diego,la jolla,ca, United States, division of biology,university of california san diego,la jolla,ca, United States, department of cellular and molecular medicine,university of california san diego,la jolla,ca,united states,stem cell program,university of california san diego,la jolla,ca,united states,institute for genomic medicine,university of california san diego,la jolla,ca, United States, division of biology,university of california san diego,la jolla,ca, United States
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Authors
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