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Contrasted Patterns of Crossover and Non-crossover at Arabidopsis thaliana Meiotic Recombination Hotspots
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نویسنده
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drouaud j. ,khademian h. ,giraut l. ,zanni v. ,bellalou s. ,henderson i.r. ,falque m. ,mézard c.
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منبع
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plos genetics - 2013 - دوره : 9 - شماره : 11
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چکیده
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The vast majority of meiotic recombination events (crossovers (cos) and non-crossovers (ncos)) cluster in narrow hotspots surrounded by large regions devoid of recombinational activity. here,using a new molecular approach in plants,called pollen-typing
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کلیدواژه
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we detected and characterized hundreds of CO and NCO molecules in two different hotspot regions in Arabidopsis thaliana. This analysis revealed that COs are concentrated in regions of a few kilobases where their rates reach up to 50 times the genome average. The hotspots themselves tend to cluster in regions less than 8 kilobases in size with overlapping CO distribution. Non-crossover (NCO) events also occurred in the two hotspots but at very different levels (local CO/NCO ratios of 1/1 and 30/1) and their track lengths were quite small (a few hundred base pairs). We also showed that the ZMM protein MSH4 plays a role in CO formation and somewhat unexpectedly we also found that it is involved in the generation of NCOs but with a different level of effect. Finally
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آدرس
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institut jean-pierre bourgin umr1318 inra-agroparistech,versailles,france,institut de biologie de l'ecole normale supérieure (ibens),cnrs,umr 8197,inserm,u 1024,paris, France, institut jean-pierre bourgin umr1318 inra-agroparistech,versailles,france,department of biological chemistry,david geffen school of medicine,university of california los angeles (ucla),los angeles,ca, United States, institut jean-pierre bourgin umr1318 inra-agroparistech,versailles,france,institut de génomique,cea 2 rue gaston crémieux,evry, France, institut jean-pierre bourgin umr1318 inra-agroparistech,versailles, France, institut jean-pierre bourgin umr1318 inra-agroparistech,versailles,france,biotek agriculture,saint pouange, France, department of plant sciences,university of cambridge,cambridge, United Kingdom, umr de génétique végétale du moulon,inra/cnrs/univ paris-sud/agroparistech,ferme du moulon,gif sur yvette, France, institut jean-pierre bourgin umr1318 inra-agroparistech,versailles, France
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Authors
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