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Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions
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نویسنده
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copsey a. ,tang s. ,jordan p.w. ,blitzblau h.g. ,newcombe s. ,chan a.c.-h. ,newnham l. ,li z. ,gray s. ,herbert a.d. ,arumugam p. ,hochwagen a. ,hunter n. ,hoffmann e.
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منبع
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plos genetics - 2013 - دوره : 9 - شماره : 12
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چکیده
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During meiosis,structural maintenance of chromosome (smc) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. while meiotic functions of the cohesin and condensin complexes have been delineated,the role of the third smc complex,smc5/6,remains enigmatic. here we identify specific,essential meiotic functions for the smc5/6 complex in homologous recombination and the regulation of cohesin. we show that smc5/6 is enriched at centromeres and cohesin-association sites where it regulates sister-chromatid cohesion and the timely removal of cohesin from chromosomal arms,respectively. smc5/6 also localizes to recombination hotspots,where it promotes normal formation and resolution of a subset of joint-molecule intermediates. in this regard,smc5/6 functions independently of the major crossover pathway defined by the mutlγ complex. furthermore,we show that smc5/6 is required for stable chromosomal localization of the xpf-family endonuclease,mus81-mms4eme1. our data suggest that the smc5/6 complex is required for specific recombination and chromosomal processes throughout meiosis and that in its absence,attempts at cell division with unresolved joint molecules and residual cohesin lead to severe recombination-induced meiotic catastrophe. © 2013 copsey et al.
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آدرس
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mrc genome damage and stability centre,university of sussex,brighton, United Kingdom, howard hughes medical institute,university of california,davis,davis,ca,united states,department of microbiology and molecular genetics,university of california,davis,davis,ca, United States, mrc genome damage and stability centre,university of sussex,brighton, United Kingdom, whitehead institute for biomedical research,cambridge,ma, United States, mrc genome damage and stability centre,university of sussex,brighton, United Kingdom, mrc genome damage and stability centre,university of sussex,brighton, United Kingdom, mrc genome damage and stability centre,university of sussex,brighton, United Kingdom, mrc genome damage and stability centre,university of sussex,brighton, United Kingdom, mrc genome damage and stability centre,university of sussex,brighton, United Kingdom, mrc genome damage and stability centre,university of sussex,brighton, United Kingdom, department of life sciences,university of warwick,coventry, United Kingdom, whitehead institute for biomedical research,cambridge,ma,united states,department of biology,new york university,new york,ny, United States, howard hughes medical institute,university of california,davis,davis,ca,united states,department of microbiology and molecular genetics,university of california,davis,davis,ca,united states,department of biology,new york university,new york,ny,united states,department of molecular and cellular biology,university of california,davis,davis,ca,united states,department of cell biology and human anatomy,university of california,davis,davis,ca, United States, mrc genome damage and stability centre,university of sussex,brighton, United Kingdom
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Authors
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