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A comprehensive map of insulator elements for the Drosophila genome
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نویسنده
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nègre n. ,brown c.d. ,shah p.k. ,kheradpour p. ,morrison c.a. ,henikoff j.g. ,feng x. ,ahmad k. ,russell s. ,white r.a.h. ,stein l. ,henikoff s. ,kellis m. ,white k.p.
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منبع
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plos genetics - 2010 - دوره : 6 - شماره : 1
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چکیده
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Insulators are dna sequences that control the interactions among genomic regulatory elements and act as chromatin boundaries. a thorough understanding of their location and function is necessary to address the complexities of metazoan gene regulation. we studied by chip-chip the genome-wide binding sites of 6 insulator-associated proteins-dctcf,cp190,beaf-32,su(hw),mod(mdg4),and gaf-to obtain the first comprehensive map of insulator elements in drosophila embryos. we identify over 14,000 putative insulators,including all classically defined insulators. we find two major classes of insulators defined by dctcf/cp190/beaf-32 and su(hw),respectively. distributional analyses of insulators revealed that particular sub-classes of insulator elements are excluded between cis-regulatory elements and their target promoters; divide differentially expressed,alternative,and divergent promoters; act as chromatin boundaries; are associated with chromosomal breakpoints among species; and are embedded within active chromatin domains. together,these results provide a map demarcating the boundaries of gene regulatory units and a framework for understanding insulator function during the development and evolution of drosophila. © 2010 nègre et al.
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آدرس
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institute for genomics and systems biology,department of ecology and evolution,university of chicago,chicago,il, United States, institute for genomics and systems biology,department of ecology and evolution,university of chicago,chicago,il, United States, institute for genomics and systems biology,department of ecology and evolution,university of chicago,chicago,il, United States, computer science and artificial intelligence lab.,massachusetts institute of technology,cambridge,ma, United States, institute for genomics and systems biology,department of ecology and evolution,university of chicago,chicago,il, United States, division of basic sciences,fred hutchinson cancer research center,seattle,wa,united states,howard hughes medical institute,seattle,wa, United States, department of biomedical engineering,state university of new york at stony brook,stony brook,ny, United States, department of biological chemistry and molecular pharmacology,harvard medical school,boston,ma, United States, department of genetics,university of cambridge,cambridge, United Kingdom, department of physiology,development,and neuroscience,university of cambridge,cambridge, United Kingdom, ontario institute for cancer research,toronto,on,canada,cold spring harbor laboratory,cold spring harbor,ny, United States, division of basic sciences,fred hutchinson cancer research center,seattle,wa,united states,howard hughes medical institute,seattle,wa, United States, computer science and artificial intelligence lab.,massachusetts institute of technology,cambridge,ma,united states,broad institute,massachusetts institute of technology,harvard university,cambridge,ma, United States, institute for genomics and systems biology,department of ecology and evolution,university of chicago,chicago,il, United States
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Authors
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