|
|
|
|
Genome-wide identification of binding sites defines distinct functions for Caenorhabditis elegans PHA-4/FOXA in development and environmental response
|
|
|
|
|
|
|
|
نویسنده
|
zhong m. ,niu w. ,lu z.j. ,sarov m. ,murray j.i. ,janette j. ,raha d. ,sheaffer k.l. ,lam h.y.k. ,preston e. ,slightham c. ,hillier l.w. ,brock t. ,agarwal a. ,auerbach r. ,hyman a.a. ,gerstein m. ,mango s.e. ,kim s.k. ,waterston r.h. ,reinke v. ,snyder m.
|
|
منبع
|
plos genetics - 2010 - دوره : 6 - شماره : 2
|
|
چکیده
|
Transcription factors are key components of regulatory networks that control development,as well as the response to environmental stimuli. we have established an experimental pipeline in caenorhabditis elegans that permits global identification of the binding sites for transcription factors using chromatin immunoprecipitation and deep sequencing. we describe and validate this strategy,and apply it to the transcription factor pha-4,which plays critical roles in organ development and other cellular processes. we identified thousands of binding sites for pha-4 during formation of the embryonic pharynx,and also found a role for this factor during the starvation response. many binding sites were found to shift dramatically between embryos and starved larvae,from developmentally regulated genes to genes involved in metabolism. these results indicate distinct roles for this regulator in two different biological processes and demonstrate the versatility of transcription factors in mediating diverse biological roles. © 2010 zhong et al.
|
|
|
|
|
آدرس
|
department of molecular cellular developmental biology,yale university,new haven,ct, United States, department of molecular cellular developmental biology,yale university,new haven,ct, United States, department of molecular biochemistry and biophysics,yale university,new haven,ct, United States, max planck institute for molecular cell biology and genetics,dresden, Germany, department of genome sciences,school of medicine,university of washington,seattle,wa, United States, department of genetics,yale university,new haven,ct, United States, department of molecular cellular developmental biology,yale university,new haven,ct, United States, huntsman cancer institute,university of utah,salt lake city,ut, United States, department of molecular cellular developmental biology,yale university,new haven,ct,united states,department of molecular biochemistry and biophysics,yale university,new haven,ct,united states,program in computational biology and bioinformatics,yale university,new haven,ct, United States, department of genome sciences,school of medicine,university of washington,seattle,wa, United States, departments of developmental biology and genetics,stanford university medical center,stanford, United States, department of genome sciences,school of medicine,university of washington,seattle,wa, United States, department of molecular and cellular biology,harvard university,cambridge,ma, United States, department of molecular cellular developmental biology,yale university,new haven,ct,united states,department of molecular biochemistry and biophysics,yale university,new haven,ct, United States, department of molecular cellular developmental biology,yale university,new haven,ct,united states,department of molecular biochemistry and biophysics,yale university,new haven,ct, United States, max planck institute for molecular cell biology and genetics,dresden, Germany, department of molecular cellular developmental biology,yale university,new haven,ct,united states,department of molecular biochemistry and biophysics,yale university,new haven,ct, United States, department of molecular and cellular biology,harvard university,cambridge,ma, United States, departments of developmental biology and genetics,stanford university medical center,stanford, United States, department of genome sciences,school of medicine,university of washington,seattle,wa, United States, department of genetics,yale university,new haven,ct, United States, department of molecular cellular developmental biology,yale university,new haven,ct,united states,department of molecular biochemistry and biophysics,yale university,new haven,ct, United States
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|