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Genome-wide screen in Saccharomyces cerevisiae identifies vacuolar protein sorting,autophagy,biosynthetic,and tRNA methylation genes involved in life span regulation
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نویسنده
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fabrizio p. ,hoon s. ,shamalnasab m. ,galbani a. ,wei m. ,giaever g. ,nislow c. ,longo v.d.
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منبع
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plos genetics - 2010 - دوره : 6 - شماره : 7 - صفحه:1 -14
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چکیده
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The study of the chronological life span of saccharomyces cerevisiae,which measures the survival of populations of nondividing yeast,has resulted in the identification of homologous genes and pathways that promote aging in organisms ranging from yeast to mammals. using a competitive genome-wide approach,we performed a screen of a complete set of approximately 4,800 viable deletion mutants to identify genes that either increase or decrease chronological life span. half of the putative short-/long-lived mutants retested from the primary screen were confirmed,demonstrating the utility of our approach. deletion of genes involved in vacuolar protein sorting,autophagy,and mitochondrial function shortened life span,confirming that respiration and degradation processes are essential for long-term survival. among the genes whose deletion significantly extended life span are acb1,cka2,and trm9,implicated in fatty acid transport and biosynthesis,cell signaling,and trna methylation,respectively. deletion of these genes conferred heat-shock resistance,supporting the link between life span extension and cellular protection observed in several model organisms. the high degree of conservation of these novel yeast longevity determinants in other species raises the possibility that their role in senescence might be conserved. © 2010 fabrizio et al.
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آدرس
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andrus gerontology center and department of biological sciences,university of southern california,los angeles,ca,united states,laboratoire de biologie moléculaire de la cellule,centre national de la recherche scientifique,ecole normale supérieure de lyon,université de lyon,lyon, France, department of genetics,stanford university,palo alto,ca, United States, laboratoire de biologie moléculaire de la cellule,centre national de la recherche scientifique,ecole normale supérieure de lyon,université de lyon,lyon, France, andrus gerontology center and department of biological sciences,university of southern california,los angeles,ca, United States, andrus gerontology center and department of biological sciences,university of southern california,los angeles,ca, United States, department of genetics,stanford university,palo alto,ca,united states,department of pharmaceutical sciences,university of toronto,toronto,on, Canada, department of genetics,stanford university,palo alto,ca,united states,department of molecular genetics,university of toronto,toronto,on, Canada, andrus gerontology center and department of biological sciences,university of southern california,los angeles,ca, United States
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Authors
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