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Competitive repair by naturally dispersed repetitive DNA during non-allelic homologous recombination
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نویسنده
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hoang m.l. ,tan f.j. ,lai d.c. ,celniker s.e. ,hoskins r.a. ,dunham m.j. ,zheng y. ,koshland d.
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منبع
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plos genetics - 2010 - دوره : 6 - شماره : 12 - صفحه:1 -18
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چکیده
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Genome rearrangements often result from non-allelic homologous recombination (nahr) between repetitive dna elements dispersed throughout the genome. here we systematically analyze nahr between ty retrotransposons using a genome-wide approach that exploits unique features of saccharomyces cerevisiae purebred and saccharomyces cerevisiae/ saccharomyces bayanus hybrid diploids. we find that dna double-strand breaks (dsbs) induce nahr-dependent rearrangements using ty elements located 12 to 48 kilobases distal to the break site. this break-distal recombination (bdr) occurs frequently,even when allelic recombination can repair the break using the homolog. robust bdr-dependent nahr demonstrates that sequences very distal to dsbs can effectively compete with proximal sequences for repair of the break. in addition,our analysis of nahr partner choice between ty repeats shows that intrachromosomal ty partners are preferred despite the abundance of potential interchromosomal ty partners that share higher sequence identity. this competitive advantage of intrachromosomal tys results from the relative efficiencies of different nahr repair pathways. finally,nahr generates deleterious rearrangements more frequently when dsbs occur outside rather than within a ty repeat. these findings yield insights into mechanisms of repeat-mediated genome rearrangements associated with evolution and cancer. © 2010 hoang et al.
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آدرس
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howard hughes medical institute and department of embryology,carnegie institution,baltimore,md,united states,department of biology,johns hopkins university,baltimore,md, United States, howard hughes medical institute and department of embryology,carnegie institution,baltimore,md,united states,department of molecular and cell biology,university of california berkeley,berkeley,ca, United States, baltimore polytechnic institute,ingenuity program,baltimore,md,united states,university of maryland,college park,md, United States, life sciences division,lawrence berkeley national laboratory,berkeley,ca, United States, life sciences division,lawrence berkeley national laboratory,berkeley,ca, United States, department of genome sciences,university of washington,seattle,wa, United States, howard hughes medical institute and department of embryology,carnegie institution,baltimore,md, United States, howard hughes medical institute and department of embryology,carnegie institution,baltimore,md,united states,department of molecular and cell biology,university of california berkeley,berkeley,ca, United States
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Authors
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