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ARGONAUTE10 and ARGONAUTE1 regulate the termination of floral stem cells through two microRNAs in Arabidopsis
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نویسنده
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ji l. ,liu x. ,yan j. ,wang w. ,yumul r.e. ,kim y.j. ,dinh t.t. ,liu j. ,cui x. ,zheng b. ,agarwal m. ,liu c. ,cao x. ,tang g. ,chen x.
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منبع
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plos genetics - 2011 - دوره : 7 - شماره : 3
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چکیده
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Stem cells are crucial in morphogenesis in plants and animals. much is known about the mechanisms that maintain stem cell fates or trigger their terminal differentiation. however,little is known about how developmental time impacts stem cell fates. using arabidopsis floral stem cells as a model,we show that stem cells can undergo precise temporal regulation governed by mechanisms that are distinct from,but integrated with,those that specify cell fates. we show that two micrornas,mir172 and mir165/166,through targeting apetala2 and type iii homeodomain-leucine zipper (hd-zip) genes,respectively,regulate the temporal program of floral stem cells. in particular,we reveal a role of the type iii hd-zip genes,previously known to specify lateral organ polarity,in stem cell termination. both reduction in hd-zip expression by over-expression of mir165/166 and mis-expression of hd-zip genes by rendering them resistant to mir165/166 lead to prolonged floral stem cell activity,indicating that the expression of hd-zip genes needs to be precisely controlled to achieve floral stem cell termination. we also show that both the ubiquitously expressed argonaute1 (ago1) gene and its homolog ago10,which exhibits highly restricted spatial expression patterns,are required to maintain the correct temporal program of floral stem cells. we provide evidence that ago10,like ago1,associates with mir172 and mir165/166 in vivo and exhibits slicer activity in vitro. despite the common biological functions and similar biochemical activities,ago1 and ago10 exert different effects on mir165/166 in vivo. this work establishes a network of micrornas and transcription factors governing the temporal program of floral stem cells and sheds light on the relationships among different ago genes,which tend to exist in gene families in multicellular organisms. © 2011 ji et al.
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آدرس
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department of botany and plant sciences,institute of integrative genome biology,university of california riverside,riverside,ca, United States, department of botany and plant sciences,institute of integrative genome biology,university of california riverside,riverside,ca, United States, gene suppression laboratory,department of plant and soil sciences and ktrdc,university of kentucky,lexington,ky, United States, rice institute,sichuan agricultural university,chengdu, China, department of botany and plant sciences,institute of integrative genome biology,university of california riverside,riverside,ca,united states,chemgen igert program,center for plant cell biology,institute of integrative genome biology,university of california riverside,riverside,ca, United States, department of botany and plant sciences,institute of integrative genome biology,university of california riverside,riverside,ca, United States, department of botany and plant sciences,institute of integrative genome biology,university of california riverside,riverside,ca,united states,chemgen igert program,center for plant cell biology,institute of integrative genome biology,university of california riverside,riverside,ca, United States, department of botany and plant sciences,institute of integrative genome biology,university of california riverside,riverside,ca, United States, state key laboratory of plant genomics and national center for plant gene research,institute of genetics and developmental biology,chinese academy of sciences,beijing, China, department of botany and plant sciences,institute of integrative genome biology,university of california riverside,riverside,ca, United States, department of botany and plant sciences,institute of integrative genome biology,university of california riverside,riverside,ca, United States, state key laboratory of plant genomics and national center for plant gene research,institute of genetics and developmental biology,chinese academy of sciences,beijing, China, state key laboratory of plant genomics and national center for plant gene research,institute of genetics and developmental biology,chinese academy of sciences,beijing, China, gene suppression laboratory,department of plant and soil sciences and ktrdc,university of kentucky,lexington,ky, United States, department of botany and plant sciences,institute of integrative genome biology,university of california riverside,riverside,ca, United States
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Authors
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